Abstract:【Objective】 This study aimed to investigate the in vivo anti-glioma activity of Esculetin (Esc) to provide experimental evidences for further study of Esculetin as a potential anti-glioma drug. 【Method】 Rat glioma C6 cell was subcutaneously injected into BALB/c nude mouse to set up the tumor-bearing mouse model. Four groups were taken in this experiment: model group

50 mg/kg Esculetin group

100 mg/kg Esculetin group and positive control group. Tumor volume

relative tumor proliferation rate

tumor weight

inhibition rate and body weight were measured to evaluate the in vivo anti-glioma activity of Esculetin. HE staining was used to identify the tumor tissue. 【Results】 Comparing with the model group

Esculetin significantly inhibited tumor volume to 1/10

and tumor weight to 1/8 of the model group in male tumor-bearing mouse

similar to the positive control drug Nimusting (ACNU)

while showed no significant anti-glioma activity in female tumor-bearing mouse.【Conclusion】 Esculetin exhibits significant anti-glioma activity on male tumor-bearing mouse

thus provides experimental evidences for further study of Esculetin as a potential anti-glioma drug.