反复种植失败患者子宫内膜“种植窗”时期催产素受体的表达

夏婷婷; 李洁; 张媛; 习羽; 欧建平

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反复种植失败患者子宫内膜“种植窗”时期催产素受体的表达

临床研究 | 更新时间：2023-11-01

- 反复种植失败患者子宫内膜“种植窗”时期催产素受体的表达
- Endometrial Expression of Oxytocin Receptor in Women with Repeated Implantation Failure During Implantation Window and in Normal Women During Menstrual Cycle
- 中山大学学报（医学科学版） 2020年41卷第4期页码：589-595
- 作者机构：
  
  1.中山大学附属第三医院生殖医学中心，广东广州 510630
  2.中山大学附属第一医院生殖医学中心，广东广州 510080
  3.上海市第十人民医院生殖医学中心，上海 200072
  4.中山大学附属第三医院产科，广东广州 510630
  5.中山大学附属第三医院不育与性医学科，广东广州 510630
- 作者简介：
  
  夏婷婷，硕士研究生，主治医师，研究方向：反复种植失败的诊治，E-mail：xiatting@mail.sysu.edu.cn
- 基金信息：
  
  国家自然科学基金(201201201412);广东省科技计划项目(200704201003);广东省医学科研基金(A2020575)
- DOI：
  中图分类号： R711.74
- 纸质出版日期：2020-07-15，
  
  收稿日期：2020-02-26，
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夏婷婷,李洁,张媛等.反复种植失败患者子宫内膜“种植窗”时期催产素受体的表达[J].中山大学学报（医学科学版）,2020,41(04):589-595.

XIA Ting-ting,LI Jie,ZHANG Yuan,et al.Endometrial Expression of Oxytocin Receptor in Women with Repeated Implantation Failure During Implantation Window and in Normal Women During Menstrual Cycle[J].Journal of Sun Yat-sen University(Medical Sciences),2020,41(04):589-595.
夏婷婷,李洁,张媛等.反复种植失败患者子宫内膜“种植窗”时期催产素受体的表达[J].中山大学学报（医学科学版）,2020,41(04):589-595. DOI：

XIA Ting-ting,LI Jie,ZHANG Yuan,et al.Endometrial Expression of Oxytocin Receptor in Women with Repeated Implantation Failure During Implantation Window and in Normal Women During Menstrual Cycle[J].Journal of Sun Yat-sen University(Medical Sciences),2020,41(04):589-595. DOI：

摘要

目的

研究催产素受体（OTR）在反复种植失败（RIF）妇女的“种植窗”时期子宫内膜组织中的表达及其与RIF的关系，并了解正常妇女月经周期子宫内膜催产素受体的表达。

方法

采用免疫组化法和RT-PCR法检测OTR在正常妇女月经周期增殖早、中、晚期和分泌早、中、晚期子宫内膜组织及RIF妇女分泌中期子宫内膜组织的表达情况，其中正常妇女分泌中期10例，其他5个时期各3例；RIF妇女分泌中期10例。

结果

RIF妇女组子宫内膜组织分泌中期OTR蛋白质和mRNA显著高于正常妇女组（

0.05）。正常妇女月经周期子宫内膜组织存在OTR/OTR mRNA的周期性表达。

结论

RIF妇女“种植窗”时期子宫内膜OTR/OTR mRNA 高表达可能与RIF发生相关。

Abstract

Objective

To investigate the expression of endometrial oxytocin receptor (OTR) during the implantation window in women with repeated implantation failure (RIF) and its relationship with RIF

and to describe the characteristic of endometrial OTR of normal women during the menstrual cycle.

Methods

Immunohistochemistry and RT-PCR were performed on the endometrial tissue from early

middle

late proliferation and secretion phase of normal women

as well as middle secretion phase of women suffering from RIF to detect the endometrial OTR expression. Ten samples of the middle secretion phase and 5 samples of the other phases each of normal women were obtained. Ten samples of RIF women were obtained during the middle secretion phase.

Results

The OTR protein and mRNA expression of RIF patients was significantly higher than that of normal women in middle secretion phase (

0.05). There was cyclic expression of OTR/OTR mRNA of the endometrial tissue of normal women.

Conclusion

The high expression of OTR/OTR mRNA during the implantation widow might participate in the occurrence of RIF.

关键词

反复种植失败催产素受体子宫内膜

Keywords

repeated implantation failureoxytocin receptorendometrium

references

Niederberger C, Pellicer A, Cohen J, et al. Forty years of IVF[J]. Fertil Steril, 2018, 110(2): 185-324.

Coughlan C, Ledger W, Wang Q, et al. Recurrent implantation failure: definition and management[J]. Reprod Biomed Online, 2014, 28(1): 14-38.

Margalioth EJ, Ben-Chetrit A, Gal M, et al. Investigation and treatment of repeated implantation failure following IVF-ET[J]. Hum Rep, 2006, 21(12): 3036-3043.

Pierzynski P, Reinheimer TM, Kuczynski W. Oxytocin antagonists may improve infertility treatment[J]. Fertil Steril, 2007, 88(1): 213.

Pierzynski P, Zbucka-Kretowska M. Uterine contractile activity at embryo transfer--as a new pharmacotherapeutic target in assisted reproduction[J]. Ginekol Pol, 2014, 85(8): 609-613.

Dittrich R, Mueller A, Oppelt PG, et al. Differences in muscarinic-receptor agonist-, oxytocin-, and prostaglandin-induced uterine contractions[J]. Fertil Steril, 2009, 92(5): 1694-1700.

Liang YL, Kuo TC, Hung KH, et al. Oxytocin antagonist for repeated implantation failure and delay of delivery[J]. Taiwan J Obstet Gynecol, 2009, 48(3): 314-316.

Chou PY, Wu MH, Pan HA, et al. Use of an oxytocin antagonist in in vitro fertilization-embryo transfer for women with repeated implantation failure: a retrospective study[J]. Taiwan J Obstet Gynecol, 2011, 50(2): 136-140.

Lan VT, Khang VN, Nhu GH, et al. Atosiban improves implantation and pregnancy rates in patients with repeated implantation failure[J]. Reprod Biomed Online, 2012, 25(3): 254-260.

Ng EH, Li RH, Chen L, et al. A randomized double blind comparison of atosiban in patients undergoing IVF treatment[J]. Hum Rep, 2014, 29(12): 2687-2694.

Kim SK, Han EJ, Kim SM, et al. Efficacy of oxytocin antagonist infusion in improving in vitro fertilization outcomes on the day of embryo transfer: A meta-analysis[J]. Clin Exp Rep Med, 2016, 43(4): 233-239.

Xia T, Liu Q, Ye Q, et al. Serum oxytocin profiles in patients with repeated implantation failure during IVF cycles[J]. Gynecol Endocrinol, 2018, 34(12): 1048-1052.

Friebe-Hoffmann U, Baston DM, Hoffmann TK, et al. The influence of interleukin-1beta on oxytocin signalling in primary cells of human decidua[J]. Regul Pept, 2007, 142(3): 78-85.

Li J, Tan Z, Li M, et al. Proteomic analysis of endometrium in fertile women during the prereceptive and receptive phases after luteinizing hormone surge[J]. Fertil Steril, 2011, 95(3): 1161-1163.

Fanchin R, Ayoubi JM. Uterine dynamics: impact on the human reproduction process[J]. Reprod Biomed Online, 2009, 18(S2): 57-62.

Arrowsmith S, Wray S. Oxytocin: its mechanism of action and receptor signalling in the myometrium[J]. J neuroendocrinol, 2014, 26(6): 356-369.

Nanetti L, Raffaelli F, Giulietti A, et al. Oxytocin, its antagonist atosiban, and preterm labor: a role for placental nitric oxide[J]. J Matern Fetal Neonatal Med, 2015, 28(5): 611-616.

Moraloglu O, Tonguc E, Var T, et al. Treatment with oxytocin antagonists before embryo transfer may increase implantation rates after IVF[J]. Reprod Biomed Online, 2010, 21(3): 338-343.

Huang QY, Rong MH, Lan AH, et al. The impact of atosiban on pregnancy outcomes in women undergoing in vitro fertilization-embryo transfer: A meta-analysis[J]. PloS one, 2017, 12(4): e0175501.

Kim SH, Pohl O, Chollet A, et al. Differential effects of oxytocin receptor antagonists, atosiban and nolasiban, on oxytocin receptor-mediated signaling in human amnion and myometrium[J]. Mol pharmacol, 2017, 91(4): 403-415.

Dawood MY, Lau M, Khan-Dawood FS. Localization and expression of oxytocin receptor and its messenger ribonucleic acid in peri-implantation phase human endometrium during control and clomiphene-treated cycles[J]. Am J Obstet Gynecol, 1999, 181(1): 50-56.

Ono M, Maruyama T, Masuda H, et al. Side population in human uterine myometrium displays phenotypic and functional characteristics of myometrial stem cells[J]. Proc Natl Acad Sci U S A, 2007, 104(47): 18700-18705.

Richter ON, Kubler K, Schmolling J, et al. Oxytocin receptor gene expression of estrogen-stimulated human myometrium in extracorporeally perfused non-pregnant uteri[J]. Mol Hum Reprod, 2004, 10(5): 339-346.

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