腹腔注射四氢姜黄素对卵清蛋白诱导过敏性哮喘小鼠的治疗作用

武音帆; 李琴; 邹进超; 毛钰蘅; 郭兴悦; 陈彦球; 杨燕

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腹腔注射四氢姜黄素对卵清蛋白诱导过敏性哮喘小鼠的治疗作用

基础研究 | 更新时间：2023-11-01

- 腹腔注射四氢姜黄素对卵清蛋白诱导过敏性哮喘小鼠的治疗作用
- Therapeutic Effects of Intraperitoneal Injection of Tetrahydrocurcumin against Allergic Asthma in Ovalbumin-induced Murine Model
- 中山大学学报（医学科学版） 2020年41卷第4期页码：525-533
- 作者机构：
  
  1.中山大学公共卫生学院，广东广州 510080
  2.广东省营养与健康重点实验室，广东广州 510080
  3.广东省营养转化工程技术研究中心，广东广州510080
  4.中山大学公共卫生学院（深圳），广东深圳518000
  5.广州市妇女儿童医疗中心耳鼻喉科，广东广州 510623
- 作者简介：
  
  武音帆，硕士在读，研究方向：膳食营养素防治过敏性气道炎症，E-mail: wuyf39@mail2.sysu.edu.cn
- 基金信息：
  
  深圳市科技创新计划(201803073000433);中山大学实验室开放基金(20180278)
- DOI：
  中图分类号： R151.2
- 纸质出版日期：2020-07-15，
  
  收稿日期：2020-04-12，
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武音帆,李琴,邹进超等.腹腔注射四氢姜黄素对卵清蛋白诱导过敏性哮喘小鼠的治疗作用[J].中山大学学报（医学科学版）,2020,41(04):525-533.

WU Yin-fan,LI Qin,ZOU Jin-chao,et al.Therapeutic Effects of Intraperitoneal Injection of Tetrahydrocurcumin against Allergic Asthma in Ovalbumin-induced Murine Model[J].Journal of Sun Yat-sen University(Medical Sciences),2020,41(04):525-533.
武音帆,李琴,邹进超等.腹腔注射四氢姜黄素对卵清蛋白诱导过敏性哮喘小鼠的治疗作用[J].中山大学学报（医学科学版）,2020,41(04):525-533. DOI：

WU Yin-fan,LI Qin,ZOU Jin-chao,et al.Therapeutic Effects of Intraperitoneal Injection of Tetrahydrocurcumin against Allergic Asthma in Ovalbumin-induced Murine Model[J].Journal of Sun Yat-sen University(Medical Sciences),2020,41(04):525-533. DOI：

摘要

目的

探讨腹腔注射四氢姜黄素（THC）对过敏性哮喘小鼠呼吸道症状和炎症反应的治疗效果。

方法

BALB/c小鼠随机分为4组，包括：正常对照组、卵清蛋白（OVA）诱导动物模型组、阳性对照组和THC治疗组。其中，阳性对照组和THC治疗组小鼠于实验第21 ~ 25天，分别经腹腔注射2.5 mg/kg 地塞米松（DEX）或20 mg/kg THC。记录小鼠激发前后体质量变化和挠鼻情况，实验结束后处死小鼠，采集肺组织和肺泡灌洗液（BALF）评估组织病理改变、检测T细胞亚型及相关细胞因子水平。

结果

哮喘小鼠经THC腹腔注射治疗后挠鼻频率降低（

0.05），两组均数之差为5.2，其95%置信区间（CI）为0.66~9.74；肺组织病理评分减少（

0.05），两组均数之差为1.6，其95%CI为0.32~2.88；杯状细胞增生引起的粘液分泌水平减轻（

0.0001），两组均数之差为9.56，其95%CI为5.05~14.07。此外

与OVA组相比，THC治疗组小鼠肺组织Th2、Th17细胞比例减少（

0.01，两组均数之差分别为1.492和2.15，其95%CI分别为0.50~2.49和0.72~3.58），BALF中白细胞介素（IL）IL-4、IL-5、IL-13和IL-17A的水平显著降低（

0.05），两组均数之差分别为5.45、4.13、5.17和2.44，其95%CI分别为1.95~8.94、1.08~7.19、0.80~9.54和-0.30~5.17。进一步比较发现THC与DEX治疗组间肺组织病理变化、Th17细胞以及IL-13和IL-17A因子水平差异无统计学意义（

0.05）。

结论

腹腔注射THC可以在短时间内有效缓解哮喘小鼠呼吸道症状和抑制炎症反应，且安全性较高，因此可能具有哮喘发作时替代性治疗手段的潜在价值。

Abstract

Objective

To study the therapeutic effects of tetrahydrocurcumin (THC) via intraperitoneal (i.p.) injection on the respiratory symptoms and inflammatory responses in asthmatic mice.

Methods

BALB/c mice were randomly divided into 4 groups

including normal control group

ovalbumin (OVA)-induced model group

positive group and THC administration group. The latter two groups were treated with 2.5 mg/kg of dexamethasone (DEX) or 20 mg/kg of THC via i.p. injection from Day 21 to Day25. The weight changes and nasal symptoms were recorded before and after OVA challenge. The mice were sacrificed at the end of the experiment and the lung tissue and broncho-alveolar lavage fluid (BALF) were collected for assessment of histopathological alterations

Th cell subsets and related cytokine level.

Results

After THC treatment via i.p. injection

the asthmatic mice’s rubbing frequencies were reduced (

0.05) with a difference of 5.2 between the two means (95% CI = 0.66 to 9.74)

lungs’ pathological scores were reduced (

0.05) with a difference between the two means being 1.6 (95% CI = 0.32 to 2.88)

mucus production induced by hyperplasia of goblet cells was alleviated (

0.0001)with a difference between the two means being 9.56 (95% CI = 5.05 to 14.07). Besides

compared with OVA group

the percentages of Th2 and Th17 cells were reduced (

0.01) with a difference between the two means being 1.49 and 2.15 (95% CI = 0.50 to 2.49 and 0.72 to 3.58)

respectively. The levels of interleukin (IL)-4

IL-5

IL-13 and IL-17A in BALF were decreased (

0.05) with a difference between the two means being 5.45

4.13

5.17 and 2.44 (95% CI = 1.95 to 8.94

1.08 to 7.19

0.80 to 9.54 and -0.30 to 5.17)

respectively. The further comparison between THC and DEX groups showed no significant difference (

0.05) in the lung pathological change

Th17 cells

IL-13

and IL-17A levels.

Conclusions

It is concluded that i.p. injection of THC could effectively alleviate the respiratory symptoms and inhibit inflammatory reaction in asthmatic mice in short time with high safety. Therefore

i.p. injection of THC has the potential value to be the alternative therapeutic strategy for asthma.

关键词

四氢姜黄素过敏性哮喘治疗腹腔注射炎症反应

Keywords

tetrahydrocurcuminallergic asthmatherapyintraperitoneal injectioninflammatory response

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