【Objective】To refer a geographical distribution rule of alpha-L-fucosidase（AFU）reference values for the health adults in China via exploring its spatial distribution trend and its correlation with geographical factors.【Methods】A total of 6564 sam? ples of AFU reference values from 66 administrative units in the years 2004-2015 were collected，male and female of which were 3701 cases（56.4%）and 2863 cases（46.3%）. A research concerning AFU reference values in whole country were calculated using methods of information content and ridge regression.【Results】AFU reference values for Chinese healthy adults were influenced by geographical factors and presented autocorrelation，and it showed eastern and northern areas were highery than western and southern areas. 【Conclusions】AFU reference values have a spatial variation and the regional disparities should be considered in practice.