【Objective】To explore the application of Da Vinci robot in laparoscopic pyeloplasty treatment for pediatric hydrone? phrosis and to improve the effect of operation and shorten the operation time effectively.【Methods】A summary and analysis was per? formed on the intraoperative cooperation about the clinical data of 14 pediatric hydronephrosis cases（16 sides）whom were performed RALP from November 2015 to July 2016. The intraoperative cooperation includes preoperative education，adequate preparation of surgical instruments，ensuring the normal operation of robot system before surgery and also，the proper body positions，the protection for patients，the establishment of aseptic barrier during operation as well as the maintenance of robot after operation.【Results】All procedures were performed via transperitoneal approach. Docking time was shortened from 40 to 20 min，operation time lasted（115± 38）min. One severe case presented with anastomasis stenosis even after PCN dilatation，and was redid dismembered pyeloplasty half a year later and the ultimate result was good. The other cases recovered well and the overall success rate was 93.75%. There were no robot failure caused by improper cooperation and no complications caused by improper nursing among the 14 pediatric hydronephrosis cases（16 sides）in pediatric hydronephrosis operations.【Conclusion】Da Vinci robot performed well in assisting laparoscopic pyelo? plasty treatment for pediatric hydronephrosis，for it leaves light surgical trauma and short duration of operation. Adequate preoperative preparation，medical staff’s formal professional technical training and professional cooperation team can not only improve the opera? tion efficiency，but also be the key to the success of the operation.