【Objective】 To investigate the expression of Pigment Epithelium-Derived Factor (PEDF) in liver of diabetic rats treated by insulin and underling mechanism. 【Methods】 High-fat diet and low dose streptozotocin (STZ)induced diabetic rats

which were randomly divided into 2 groups: diabetic rats (n=6)

diabetic rats treated with NPH insulin (n = 6)

with 6 normal animals as control. After three-week intervention

protein expressions of PEDF

tumor necrosis factor-α (TNF-α)

matrix metalloproteinase 2 (MMP2) in liver were measured by Western blotting. 【Results】 Insulin decreased fasting blood glucose

serum triglycerides and liver triglycerides in diabetic rats. Significant lipid deposition was reversed in livers of diabetic rats treated by insulin. Compared to normal rats

untreated diabetic rats had higher TNF-α

MMP2 protein expressions and lower PEDF protein expression. The response to insulin therapy in diabetic rats showed downregulated protein expressions of TNF-α and MMP2

upregulated PEDF in liver. 【Conclusion】 The upregulation of PEDF protein expression by insulin may be responsible for the anti-inflammatory action of insulin

which maybe one of the mechanisms that insulin ameliorates triglycerides deposition in liver.