【Objective】To analyze the induction of glucose transporter 4（GLUT4）by Fusobacterium nucleatum（Fn）in esophageal squamous cell carcinoma（ESCC）and its correlation with clinicopathologic features and 5-year survival，and to explore its clinical significance and prognostic value.【Methods】Paraffin-embedded specimens of esophageal squamous cell carcinoma were collected from 96 patients with this disease from January 2014 to March 2015 at Anyang Cancer Hospital. RNAscope and immunohistochemistry were used to detect the expression of Fn and GLUT4 in Esophageal squamous cell carcinoma tissues，and to analyze the induced effect of Fn on GLUT4 and its correlation with clinicopathologic features. Survival curves were drawn by Kaplan-Meier survival analysis and correlation between GLUT4-induced effect of Fn and survival time was analyzed by Log-rank test.【Results】There were red granules in the cytoplasm of ESCC ，which were positive for Fn infection，brown granules in the plasma membrane of ESCC were positive for GLUT4 expression，and the expression of GLUT4 was consistent with the Fn infection（P < 0.05）. At the same time，the positive expression of GLUT4 induced by Fn was significantly correlated with gender ，smoking，drinking，degree of differentiation，depth of invasion，lymph node metastasis and clinical stage（P < 0.05）. The 5-year survival rate and median survival time in the positive group were significantly lower than those in the negative group（P < 0.05）.【Conclusions】Long- term smoking and drinking will lead to bad oral environment. Fn is more easily infected and planted in this environment ，which can induce the high expression of GLUT4 in plasma membrane of cancer cells，enhance glucose metabolism，and promote the malignant progression of ESCC. Effective removal of Fn and inhibition of GLUT4 expression may provide a new strategy and effective treatment for esophageal squamous cell carcinoma.