To compare the effectiveness of digital PCR and blood culture for detecting methicillin-resis⁃ tant Staphylococcus aureus（MRSA）bloodstream infection in children.【Methods】Blood samples from 246 child patients from October 2017 to September 2019 were obtained simultaneously for digital PCR and blood culture to detect MRSA infection. SPSS 18.0 software was used for statistical analysis.【Results】Among the 246 blood samples，69 were found positive，with 53 positive by both digital PCR and blood culture ，11 positive by digital PCR and 5 positive by blood culture. Another 177 blood samples were identified as negative by both methods. The overall agreement between the two tests was 93.50%（230/246）. The average time-to-positivity（TTP）of blood culture was 48 h and time to obtain test results for digital PCR averaged 2.5 h. Considering the blood culture as the gold standard，the sensitivity，specificity，positive predictive value and negative predictive value of digital PCR were 91.40%（53/58），94.15%（177/188），82.81%（53/64）， 97.25%（177/182），respectively. ROC curve showed that the area under the curve of the digital PCR was 93% ，which revealed higher diagnostic value. 【Conclusion】In the diagnosis of MRSA bloodstream infection in children，compared with blood culture，digital PCR is simpler，more rapid，more sensitive and more specific. Digital PCR helps rapid diagnosis and early precise treatment for MRSA infection.