西南交通大学生命科学与工程学院,四川 成都 610031
熊伟,硕士生,研究方向:中药药理学,E-mail: wei_xiong@my.swjtu.edu.cn
纸质出版日期:2023-01-20,
收稿日期:2022-10-14,
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熊伟,胡林,许青等.基于“心与小肠相表里”探讨阿尔茨海默病小鼠全肠道菌群移植对回肠肠道微环境的影响[J].中山大学学报(医学科学版),2023,44(01):63-70.
XIONG Wei,HU Ling,XU Qing,et al.Probing the Impact of Whole Intestinal Microbiota Transplantation from Alzheimer's Disease Model Mice on the Ileal Microenvironment Based on the “Interior-Exterior Relationship Exists Between Heart and Small Intestine”[J].Journal of Sun Yat-sen University(Medical Sciences),2023,44(01):63-70.
熊伟,胡林,许青等.基于“心与小肠相表里”探讨阿尔茨海默病小鼠全肠道菌群移植对回肠肠道微环境的影响[J].中山大学学报(医学科学版),2023,44(01):63-70. DOI: 10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2023.0109.
XIONG Wei,HU Ling,XU Qing,et al.Probing the Impact of Whole Intestinal Microbiota Transplantation from Alzheimer's Disease Model Mice on the Ileal Microenvironment Based on the “Interior-Exterior Relationship Exists Between Heart and Small Intestine”[J].Journal of Sun Yat-sen University(Medical Sciences),2023,44(01):63-70. DOI: 10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2023.0109.
目的
2
在“心与小肠相表里”中医理论指导下,探讨阿尔茨海默病(AD)模型小鼠全肠道菌群移植,是否对正常小鼠回肠肠道微环境的影响更显著。
方法
2
将14只6月龄无特定病原体的雄性
APP/PS1
双转基因AD模型小鼠的全肠道菌群,移植至6只混合抗生素处理过的同月龄同背景正常C57BL/6J小鼠肠道内,移植14 d后再定殖14 d,观察小鼠回肠和结肠组织病理变化,并采用16S核糖体RNA(16S rRNA)基因测序技术分析小鼠回肠和结肠菌群。
结果
2
AD小鼠全肠道菌群移植到正常小鼠后,病理分析表明仅回肠组织出现粘膜层破坏和隐窝腺上皮细胞变性、坏死脱落;测序分析发现,仅回肠菌群的种类数量(
P
<
0.01)、Chao1指数(
P
<
0.01)和Simpson指数(
P
<
0.01)降低且菌群组成和AD模型小鼠更相似。
结论
2
AD模型小鼠全肠道菌群移植对正常小鼠回肠菌群多样性及组成的影响显著高于结肠菌群,同时导致回肠粘膜的病理损伤,表明回肠肠道微环境可能和AD的发生发展关系更加密切,与“心与小肠相表里”中医理论高度切合。
Objective
2
To investigate whether the whole intestinal microbiota transplantation in Alzheimer's disease (AD) model mice has more significant effects on ileum intestinal microenvironment in normal mice under the guidance of the theory of traditional Chinese medicine that "interior-exterior relationship exists between the heart and small intestine".
Methods
2
The whole intestinal microbiota of fourteen 6-month-old specific pathogen free male
APP
/
PS1
double-transgenic AD model mice was transplanted into the gut of six normal C57BL/6J mice of the same age and background treated with mixed antibiotics for 14 days. Then, after 14 days of normal rearing, the mice were sacrificed. Next, the pathological changes in the ileum and colon were observed, and the composition and diversity of the ileal and colonic microbiota was analyzed by sequencing.
Results
2
After the whole intestinal microbiota of AD mice was transplanted into normal mice, pathological analysis showed that only the ileum tissue had mucosal damage and crypt gland epithelial cell degeneration, necrosis, and shedding. Moreover, the microbiota analysis found that only the number of genera (
P
<
0.01), Chao1 index (
P
<
0.01) and Simpson index of ileal microbiota in normal mice decreased (
P
<
0.01), and the composition of intestinal microbiota was quite similar to that of AD model mice.
Conclusion
2
Under the effect of whole gut microbiota transplantation in AD mice, the diversity and composition of ileal microbiota change more than that of colonic microbiota in normal mice, and at the same time, it results in pathological damage to the ileal mucosa, indicating that the ileal microenvironment may be more closely related to the occurrence and development of AD, which is highly consistent with the traditional Chinese medicine theory of "interior-exterior relationship between heart and small intestine".
中医理论阿尔茨海默病全肠道菌群移植回肠肠道微环境结肠肠道微环境
traditional Chinese medicine theoryAlzheimer's diseasewhole intestinal microbiota transplantationileal microenvironmentcolonic microenvironment
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