6种沙眼衣原体蛋白抗体在输卵管性不孕患者中的表达及检测价值

刘明娜; 齐蔓莉; 陈小红; 谭金凤; 张丹; 刘雨嫣; 韩建德; 马春光

doi:10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2023.0121

您当前的位置：

首页 >

文章列表页 >

6种沙眼衣原体蛋白抗体在输卵管性不孕患者中的表达及检测价值

临床研究 | 更新时间：2024-02-19

- 6种沙眼衣原体蛋白抗体在输卵管性不孕患者中的表达及检测价值
- Expression and Detection Value of 6 Chlamydia Trachomatis Protein Antibodies in Tubal Factor Infertility
- 中山大学学报（医学科学版） 2023年44卷第1期页码：150-158
- 作者机构：
  
  1.中山大学附属第一医院皮肤科，广东广州 510080
  2.天津医科大学总医院皮肤性病科，天津301500
  3.中山大学附属第一医院妇科，广东广州 510080
  4.中山大学附属第一医院生殖中心，广东广州 510080
- 作者简介：
  
  刘明娜，硕士生，研究方向：沙眼衣原体感染，E-mail：liumn9@mail2.sysu.edu.cn
- 基金信息：
  
  广州市科技计划项目(201807010081)
- DOI：10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2023.0121
  中图分类号： R759
- 纸质出版日期：2023-01-20，
  
  收稿日期：2022-06-07，
扫描看全文
刘明娜,齐蔓莉,陈小红等.6种沙眼衣原体蛋白抗体在输卵管性不孕患者中的表达及检测价值[J].中山大学学报（医学科学版）,2023,44(01):150-158.

LIU Ming-na,QI Man-li,CHEN Xiao-hong,et al.Expression and Detection Value of 6 Chlamydia Trachomatis Protein Antibodies in Tubal Factor Infertility[J].Journal of Sun Yat-sen University(Medical Sciences),2023,44(01):150-158.
刘明娜,齐蔓莉,陈小红等.6种沙眼衣原体蛋白抗体在输卵管性不孕患者中的表达及检测价值[J].中山大学学报（医学科学版）,2023,44(01):150-158. DOI： 10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2023.0121.

LIU Ming-na,QI Man-li,CHEN Xiao-hong,et al.Expression and Detection Value of 6 Chlamydia Trachomatis Protein Antibodies in Tubal Factor Infertility[J].Journal of Sun Yat-sen University(Medical Sciences),2023,44(01):150-158. DOI： 10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2023.0121.

摘要

目的

研究不同类型沙眼衣原体蛋白在输卵管性不孕中的致病作用，预测具有临床检测价值的沙眼衣原体蛋白抗体。

方法

本研究共纳入输卵管性不孕组（TFI）58例，正常生育组（FC）41例，非输卵管性不孕组（IFC）18例。血清检测CT-IgG ELISA试剂盒检测三组人群血清中CT-IgG的表达情况；利用前期获得表达、纯化的6种沙眼衣原体蛋白，建立这6种沙眼衣原体蛋白抗体的ELISA检测方法，分别检测6种沙眼衣原体蛋白抗体在TFI组、FC组和IFC组血清中的表达情况；对三组研究对象6种沙眼衣原体蛋白抗体的OD值进行统计描述，以CT-IgG的检测结果（阳/阴）为参考标准，绘制6种沙眼衣原体蛋白抗体的受试者工作特征曲线，利用约登指数确定各抗体的截断值。以TFI作为参考类，将FC、IFC分别与参考类建立两个无序多分类logistic回归模型，探究沙眼衣原体蛋白抗体、年龄预测TFI、FC和IFC的价值，并利用后退法筛选变量。

结果

各组研究对象CT376和CT443抗体OD值不全相同，CT-IgG阳性率不全相同。经两两比较发现，TFI组的CT376抗体OD值高于FC组（0.86

. 0.60，

=0.026）。TFI组的CT376抗体OD值高于IFC组（0.86

. 0.64，

=0.026）。IFC组CT443抗体OD值高于TFI组（0.59

. 0.34，

=0.036），高于FC组（0.59

. 0.30，

=0.02）。经过比较，CT376抗体的阳性率在三组间不全相同（

0.05）；无序多分类logistic回归分析结果表明：TFI与FC建立的无序多分类logistic回归分析结果显示，CT-IgG ［

0.001，OR=0.084，95%CI（0.025，0.284）］、CT376抗体［

=0.068， OR=0.359，95%CI（0.120，1.078）］。CT-IgG是输卵管性不孕发生的独立危险因素，CT376抗体尚不能成为输卵管性不孕的独立危险因素；TFI与IFC建立的无序多分类logistic回归分析显示，在不孕的患者中，CT-IgG ［

0.05，OR=0.194，95%CI（0.046，0.817）］、CT376 抗体［

0.05，OR=0.176，95%CI（0.038，0.818）］和CT381 抗体［

0.05，OR=0.112，95%CI（0.016，0.796）］是输卵管性不孕发生的独立危险因素。

结论

CT376抗体在输卵管性不孕患者中的表达情况高于正常生育及非输卵管性不孕的人群，提示CT引起的输卵管性不孕或许与CT376有关。检测CT-IgG和CT376血清抗体可能对CT引起的输卵管性不孕有预测价值。

Abstract

Objective

To further study the pathogenic role of different types of

Chlamydia trachomatis

（CT） proteins in tubal factor infertility， evaluate the clinical detection value of

Chlamydia trachomatis

protein antibody in predicting tubal factor infertility.

Methods

A total of 58 cases of tubal factor infertility （TFI）， 41 cases of fertile controls （FC） and 18 cases of infertile controls （IFC） were included. For serum detection， first， CT-IgG ELISA kit was used to detect the expression of CT-IgG in serum of three groups of people； then， 6 kinds of

Chlamydia trachomatis

proteins were expressed and purified in the early stage to establish the antibody test for these proteins， and ELISA detection method was used to detect the expression of their antibodies in the serum of TFI group， FC group and IFC group， respectively； and finally， the antibody OD value of the 6 kinds of

Chlamydia trachomatis

proteins in the three groups of subjects were statistically described， and CT-IgG was used as the reference standard to draw the receiver operating characteristic curve （ROC curve） of each CT antibody. The Youden Index determines the cutoff value for each antibody. Taking TFI as the reference class， two disordered multiple classification logistic regression models were established with the FC and IFC groups， respectively； and the reference class was used to explore the value of various antibodies and age in predicting TFI， FC and IFC of

Chlamydia trachomatis

. The back-off method was used to screen the variables.

Results

The OD value of CT376 antibody in the TFI group was higher than that in the FC group （0.86

vs.

0.60，

=0.026）. The CT376 antibody OD value in the TFI group was higher than that in the IFC group （0.86

vs.

0.64，

=0.026）. The CT443 antibody OD value in the IFC group was higher than that in the TFI group （0.59

vs.

0.34，

=0.036） and higher than that in the FC group （0.59

vs.

0.30，

=0.02）. The multiple classification logistic regression analysis established between TFI and FC showed that CT-IgG ［

0.001， OR=0.084， 95%CI （0.025， 0.284）］， CT376 antibody ［

=0.068， OR=0.359， 95%CI （0.120， 1.078）］. CT-IgG is an independent risk factor for tubal infertility， and CT376 antibody cannot be an independent risk factor for tubal infertility. The multiple classification logistic regression analysis established between TFI and IFC showed that among infertile patients， CT-IgG ［

0.05，

OR=0.194， 95%CI （0.046， 0.817）］， CT376 antibody ［

0.05， OR=0.176， 95%CI （0.038， 0.818）］ and CT381 antibody ［

0.05， OR=0.112， 95%CI （ 0.016， 0.796）］ were independent risk factors for tubal infertility.

Conclusion

The expression of CT376 antibody in tubal infertility patients is higher than that in fertile and infertile controls， suggesting that CT-induced tubal factor infertility may be related to CT376. CT-IgG， and CT376 antibodies are meaningful in predicting CT-induced tubal factor infertility.

关键词

沙眼衣原体输卵管性不孕沙眼衣原体抗体

Keywords

Chlamydia trachomatistubal factor infertilityChlamydia trachomatis antibody

references

de la Maza LM， Zhong G， Brunham RC. Update on Chlamydia trachomatis vaccinology［J］. Clin Vaccine Immunol， 2017， 24（4）： e00543-16.

杨晗，陈文娟，赵江红，等. 中期妊娠引产706例患者性传播疾病和阴道感染状况分析［J］.广东医学， 2018， 39（S2）： 156-158.

Yang H， Chen WJ， Zhao JH， et al. Analysis of sexually transmitted diseases and vaginal infections in 706 cases of mid term pregnancy induced labor［J］. J Guangdong ， 2018， 39（S2）： 156-158.

Rowley J， Vander Hoorn S， Korenromp E， et al. Chlamydia， gonorrhoea， trichomoniasis and syphilis： global prevalence and incidence estimates， 2016［J］. Bulletin of the World Health Organization， 2019， 97（8）： 548-562P.

Mahmud NU. Incidence of genital Chlamydia trachomatis infection in sexually active women attending the Mymensingh Medical College Hospital［D］. Bangladesh： Department of Microbiology Mymensingh Medical College Mymensingh， 2010.

徐天程，陈建林，施晓波. 沙眼衣原体泌尿生殖道感染的实验室诊断研究进展［J］. 临床检验杂志， 2019， 37（4）： 293-296.

Xu TC， Chen JL， Shi XB. Chlamydia trachomatis research progress of laboratory diagnosis of urinary tract infection ［J］. J Clin Exam， 2019， 5 （4）： 293-296.

Horner PJ， Anyalechi GE， Geisler WM. What can serology tell us about the burden of infertility in women caused by Chlamydia？［J］. J Infect Dis， 2021， 224（12 Suppl 2）： S80-S85.

Rodgers AK， Budrys NM， Gong S， et al. Genome-wide identification of Chlamydia trachomatis antigens associated with tubal factor infertility［J］. Fertil Steril， 2011， 96（3）： 715-721.

Budrys NM， Gong S， Rodgers AK， et al. Chlamydia trachomatis antigens recognized in women with tubal factor infertility， normal fertility， and acute infection［J］. Obstet Gynecol， 2012， 119（5）： 1009-1016.

Chlamydia trachomatis IgG ELISA［Z］. https：//www.ibl-international.com/media/mageworx/downloads/attachment/file/r/e/re57011_ifu_eu_multi_chlamydia_trachomatis_igg_elisa_vn-2018-05-16.pdf

Centers for Disease Control and Prevention. Sexually Transmitted Disease Surveillance 2019［M/OL］. Atlanta：U. S. Department of Health and Human Services，2021（2022-04-05）［2022-06-06］. https：//www.cdc.gov/std/chlamydia/stdfact-chlamydia-detailed.htmhttps://www.cdc.gov/std/chlamydia/stdfact-chlamydia-detailed.htm

岳晓丽，龚向东，李婧，等. 2015—2019年中国性病监测点生殖道沙眼衣原体感染流行病学特征［J］. 中华皮肤科杂志， 2020， 53（8）： 596-601.

Yue XL， Gong XD， Li J， et al. Epidemiologic features of genital Chlamydia trachomatis infection at national sexually transmitted disease surveillance sites in China， 2015—2019［J］. Chin J Dermatol， 2020， 53（8）： 596-601.

Kessler LM， Craig BM， Plosker SM， et al. Infertility evaluation and treatment among women in the United States［J］. Fertil Steril， 2013， 100（4）： 1025-1032.

Woodhall SC， Gorwitz RJ， Migchelsen SJ， et al. Advancing the public health applications of Chlamydia trachomatis serology［J］. Lancet Infect Dis， 2018，18（12）： e399-e407.

Chernesky MA. The laboratory diagnosis of Chlamydia trachomatis infections［J］. Can J Infect Dis Med Microbiol， 2005， 16（1）： 39-44.

Wang SP， Grayston JT. Human serology in Chlamydia trachomatis infection with microimmunofluorescence［J］. J Infect Dis， 1974， 130（4）： 388-397.

Saadouni A， Tbai N， Takourt B. Comparison of two techniques for serological diagnosis of chlamydial infections： MIF and immunoblotting［J］. Ann Biol Clin （Paris）， 2013， 71（6）： 663-666.

Johnson AM， Horner P. A new role for Chlamydia trachomatis serology？［J］. Sex Transm Infect， 2008， 84（2）： 79-80.

Emmanuel F. Serum levels of anti-Chlamydial antibody in fertile and infertile women in selected health institusions in Ios［D］. Emmanuel Jos University Teaching Hospital， 2015.

den Hartog JE， Land JA， Stassen FRM， et al. Serological markers of persistent C. trachomatis infections in women with tubal factor subfertility［J］. Hum Reprod， 2005， 20（4）： 986-990.

Horner PJ， Flanagan H， Horne AW. Is there a hidden burden of disease as a result of epigenetic epithelial-to-mesenchymal transition followingchlamydia trachomatis genital tract infection？［J］. J Infect Dis， 2021， 224（12 Suppl 2）： S128-S136.

Coppus SFPJ， Land JA， Opmeer BC， et al. Chlamydia trachomatis IgG seropositivity is associated with lower natural conception rates in ovulatory subfertile women without visible tubal pathology［J］. Hum Reprod， 2011， 26（11）： 3061-3067.

Steiner AZ， Diamond MP， Legro RS， et al. Chlamydia trachomatis immunoglobulin G3 seropositivity is a predictor of reproductive outcomes in infertile women with patent fallopian tubes［J］. Fertil Steril， 2015， 104（6）： 1522-1526.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

暂无数据