1.广州中医药大学附属宝安中医院//广州中医药大学第七临床医学院,广东 深圳 518133
2.广州中医药大学第一附属医院, 广东 广州510405
3.广州中医药大学第二附属医院,广东 广州 510120
李晶晶, 博士,副主任医师,研究方向:针灸对损容性皮肤病的机制研究,E-mail:lijingjing85@gzucm.edu.cn
纸质出版日期:2023-03-20,
收稿日期:2022-08-01,
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李晶晶,曾婧纯,梁雪松等.岭南火针通过调控Hippo-YAP信号通路抑制白癜风模型小鼠黑素细胞凋亡[J].中山大学学报(医学科学版),2023,44(02):244-253.
LI Jing-jing,ZENG Jing-chun,LIANG Xue-song,et al.Lingnan Fire-needle on Inhibiting Melanocyte Apoptosis in Vitiligo Model Mice by Regulating Hippo-YAP Signaling Pathway[J].Journal of Sun Yat-sen University(Medical Sciences),2023,44(02):244-253.
李晶晶,曾婧纯,梁雪松等.岭南火针通过调控Hippo-YAP信号通路抑制白癜风模型小鼠黑素细胞凋亡[J].中山大学学报(医学科学版),2023,44(02):244-253. DOI: 10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2023.0208.
LI Jing-jing,ZENG Jing-chun,LIANG Xue-song,et al.Lingnan Fire-needle on Inhibiting Melanocyte Apoptosis in Vitiligo Model Mice by Regulating Hippo-YAP Signaling Pathway[J].Journal of Sun Yat-sen University(Medical Sciences),2023,44(02):244-253. DOI: 10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2023.0208.
目的
2
基于Hippo-YAP信号通路探讨岭南火针对氢醌诱导的氧化应激后白癜风模型的影响。
方法
2
将C57BL/6 小鼠随机分为空白对照组(Control)、模型对照组(HQ)、HQ+卤米松组 (Halometasone)、HQ+岭南火针组(FA),每组8只。采用氢醌(HQ)建立白癜风模型。通过脱色评分、HE 染色、Masson-Fontana法观察皮肤病理学改变;ELISA 法检测各组血浆酪氨酸酶(TYR)、丙二醛(MDA)含量及血清单胺氧化酶(MAO)活性;Western-blot检测皮肤组织YAP1、TP73蛋白表达量;PCR法检测
Yap1、Tp73
表达水平。
结果
2
与Control组相比,造模后,小鼠表皮层和真皮层明显增厚,含黑素细胞毛囊数、基底层黑素细胞数、含黑素颗粒表皮细胞数明显减少,脱色评分明显提高,TYR水平下降,MDA、MAO水平上升,
Yap1、Tp73
低表达(
P
<0.01)。经过4周治疗后,Halometasone组、FA组真皮层变薄,皮肤脱色评分下降,黑素细胞毛囊数、基底层黑素细胞数、含黑素颗粒表皮细胞数增加(
P
<0.05),两个治疗组TYR水平与HQ组相比显著升高(
P
<0.01),MDA含量与HQ组相比显著下降(
P
<0.01),FA组MAO活性值与HQ组相比下调(
P
<0.05)。FA组
Yap1
和
Tp73
表达与HQ组相比明显上调(
P
<0.01),且趋势强于Halometasone组(
P
<0.05)。
结论
2
岭南火针可能通过激活 Hippo-YAP通路保护黑素细胞免受氢醌诱导的氧化应激损伤,降低氧化应激产物含量及其活性,提升黑素细胞的合成及功能,从而起到促进白斑复色的作用。
Objective
2
To observe the effects of fire-needle of Lingnan on the vitiligo model after hydroquinone-induced oxidative stress based on the Hippo-YAP signaling pathway.
Methods
2
C57BL/6 mice were randomly divided into normal group (Control), model group (HQ), HQ+fire-needle group (FA), and positive control group (Halometasone), with 8 mice in each group. The vitiligo model was prepared by hydroquinone (HQ). The skin pathological changes were observed by depigmentation score, HE staining and Masson-Fontana. Elisa was used to detect the levels of tyrosinase (TYR), malondialdehyde (MDA) and monoamine oxidase (MAO).Western-blot and PCR were used to detect the expression of
Yap1
and
Tp73
among the groups.
Results
2
Compared with the control group, the epidermis and dermis were significantly thicker. The number of melanocyte hair follicles, basal melanocytes, epidermal cells containing melanin granules were significantly decreased, and the depigmentation score was significantly reduced(
P
<0.01). The level of TYR decreased, and the levels of MDA and MAO increased after modeling(
P
<0.01). The expression of
Yap1
and
Tp73
were significantly reduced (
P
<
0.01). The dermis became thinner in the halometasone and FA group after treatment of 4 weeks. The number of melanocyte hair follicles, basal melanocytes, epidermal cells containing melanin granules increased (
P
<
0.05). Compared with that of the HQ group, the level of TYR in the halometasone group and FA group was significantly increased (
P
<
0.01). The levels of MDA and MAO in the FA group were decreased (
P
<
0.05). The expressions of
Yap1
and
Tp73
in the FA group were significantly increased (
P
<
0.01), and their effects were better than those in the Halometasone group (
P
<
0.05).
Conclusions
2
Fire-needle of Lingnan protects melanocytes from oxidative stress by activating the Hippo-YAP pathway. It enhances the synthesis and function of melanocytes and promotes repigmentation by reducing the content and activity of oxidative stress products.
岭南火针Hippo-YAP信号通路氧化应激白癜风黑素细胞
fire-needle of lingnanHippo-YAP signaling pathwayoxidative stressvitiligomelanocytes
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