黄芩多糖通过调节JAK2/STAT3通路和IL-23/IL-17炎性轴改善DSS诱导的UC模型小鼠的炎症
Scutellaria Baicalensis Polysaccharides Alleviates the Inflammation of DSS-induced UC Model Mice by Regulating JAK2/STAT3 Pathway and IL-23/IL-17 Inflammatory Axis
- 中山大学学报（医学科学版） 2023年44卷第3期 页码：423-429
- 作者机构：
1.锦州医科大学附属第一医院消化内科，辽宁 锦州121000
2.葫芦岛市中心医院消化内科，辽宁 葫芦岛125000
- 作者简介：
马 杰，硕士生，研究方向：消化道肿瘤，E-mail：1687245388@qq.com
- 基金信息：辽宁省科学技术基金(2020-MS-295)
DOI：10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2023.0308
中图分类号： R285.5纸质出版日期：2023-05-20，
收稿日期：2022-11-03，
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马杰,陈韵之,田蕾.黄芩多糖通过调节JAK2/STAT3通路和IL-23/IL-17炎性轴改善DSS诱导的UC模型小鼠的炎症[J].中山大学学报（医学科学版）,2023,44(03):423-429.
MA Jie,CHEN Yun-zhi,TIAN Lei.Scutellaria Baicalensis Polysaccharides Alleviates the Inflammation of DSS-induced UC Model Mice by Regulating JAK2/STAT3 Pathway and IL-23/IL-17 Inflammatory Axis[J].Journal of Sun Yat-sen University(Medical Sciences),2023,44(03):423-429.
马杰,陈韵之,田蕾.黄芩多糖通过调节JAK2/STAT3通路和IL-23/IL-17炎性轴改善DSS诱导的UC模型小鼠的炎症[J].中山大学学报（医学科学版）,2023,44(03):423-429. DOI： 10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2023.0308.
MA Jie,CHEN Yun-zhi,TIAN Lei.Scutellaria Baicalensis Polysaccharides Alleviates the Inflammation of DSS-induced UC Model Mice by Regulating JAK2/STAT3 Pathway and IL-23/IL-17 Inflammatory Axis[J].Journal of Sun Yat-sen University(Medical Sciences),2023,44(03):423-429. DOI： 10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2023.0308.
摘要
目的
2
研究黄芩多糖对溃疡性结肠炎小鼠（UC）肠道炎症的影响及其机制。
方法
2
选用C57BL/6小鼠随机分为正常组、模型组、美沙拉嗪组以及黄芩多糖低、中、高剂量组（10只/组）。观察小鼠日常状态并记录疾病活动指数（DAI）评分。酶联免疫吸附测定（ELISA）法检测血清中细胞因子白细胞介素-6、17、23（IL-6、IL-17、IL-23）的表达。处死小鼠，HE观察小鼠肠黏膜损伤情况记录结肠组织损伤指数（TDI）评分，Western blot法和免疫组化法检测Janus激酶2（JAK2）、信号转导因子和转录激活因子（STAT3）、p-JAK2、p-STAT3蛋白的表达水平。
结果
2
与空白组相比，模型组小鼠DAI评分升高
，
血清中IL-6、IL-17、IL-23含量升高，结肠组织p-JAK2/JAK2、p-STAT3/STAT3比值升高，p-JAK2、p-STAT3表达量升高。与模型组相比，各给药组小鼠DAI评分降低；血清中IL-6、IL-17、IL-23含量降低，TDI评分降低
，
结肠组织p-JAK2/JAK2、p-STAT3/STAT3比值降低，p-JAK2、p-STAT3表达量降低。
结论
2
黄芩多糖可能通过JAK2/STAT3通路和IL-23/IL-17炎性轴改善UC小鼠的炎症。
Abstract
Objective
2
To investigate the effect and mechanism of Scutellaria Baicalensis polysaccharides on intestinal immunity in mice with ulcerative colitis （UC）.
Methods
2
C57BL/6 mice were randomly assigned to blank group， model group， mesalazine group and Scutellaria Baicalensis polysaccharides low-dose， medium-dose and high-dose groups （
n
=10 mice/group）. The daily status of mice was observed and the disease activity index （DAI） score was recorded. The expression of cytokines including IL-6， IL-17 and IL-23 in serum was detected by ELISA. After the mice were sacrificed， HE was used to observe the intestinal mucosal damage in mice， and the colonic tissue damage index （TDI） score was recorded. Western blot and immunohistochemistry were used to detect the expression levels of Janus kinase 2 （JAK2）， signal transducer and activator of transcription （STAT3）， p-JAK2 and p-STAT3 proteins.
Results
2
Compared with the blank group， the DAI score of the model group was significantly increased， the contents of IL-6， IL-17 and IL-23 in serum were significantly increased， the ratio of p-JAK2/JAK2 and p-STAT3/STAT3 in colon tissue was significantly increased， and the expression levels of p-JAK2 and p-STAT3 were significantly increased. Compared with the model group， the DAI score of mice in each administration group was significantly decreased. The contents of IL-6， IL-17 and IL-23 in serum were decreased， the TDI score was decreased， the ratio of p-JAK2/JAK2 and p-STAT3/STAT3 in colon tissue was decreased， and the expression of p-JAK2 and p-STAT3 was decreased.
Conclusion
2
Scutellaria Baicalensis polysaccharides may improve the inflammatory effect of UC mice through JAK2/STAT3 pathway and IL-23/IL-17 inflammatory axis.
关键词
黄芩多糖溃疡性结肠炎IL-17IL-23JAK2/STAT3通路
Keywords
Scutellaria Baicalensis polysaccharideulcerative colitisIL-17IL-23JAK2/STAT3 pathway
references
Zhu Q， Zheng P， Chen X， et al. Andrographolide presents therapeutic effect on ulcerative colitis through the inhibition of IL-23/IL-17 axis［J］. Am J Transl Res， 2018， 10（2）：465-473.
Imam T， Park S， Kaplan MH， et al. Effector T helper cell subsets in inflammatory bowel diseases［J］. Front Immunol， 2018， 9：1212.
Noviello D， Mager R， Roda G， et al. The IL23-IL17 immune axis in the treatment of ulcerative colitis： successes， defeats， and ongoing challenges［J］. Front Immunol， 2021， 12：611256.
Lin H， Honglang L， Weifeng L， et al. The mechanism of alopolysaccharide protecting ulceralive colitis［J］. Biomed Pharmacother， 2017， 88：145-150.
Chu XQ， Wang J， Chen GX， et al. Overexpression of microRNA-495 improves the intestinal mucosal barrier function by targeting STAT3 via inhibition of the JAK/STAT3 signaling Pathway in a mouse model of ulcerative colitis［J］. Pathol Res Pract， 2018， 214（1）：151-162.
Raychaudhuri SK， Abria C， Raychaudhuri SP. Regulatory role of the JAK STAT kinase signalling system on the IL-23/IL-17 cytokine axis in psoriatic arthritis［J］. Ann Rheum Dis， 2017， 76（10）：e36.
崔莉，宁青，张润桐，等.黄芩多糖提取条件优化方法及其对溃疡性结肠炎小鼠的疗效研究［J］.山东中医杂志，2020，39（9）：993-1000.
Cui L， Ning Q， Zhang RT， et al. Optimization of extraction conditions of Baicalin polysaccharide and its effect on mice with ulcerative colitis［J］.Shandong J Tradit Chin Med， 2020， 39（9）；993-1000.
Ungaro R， Mehandru S， Allen PB， et al. Ulcerative colitis［J］. Lancet， 2017， 389（10080）：1756-1770.
Moschen AR， Tilg H， Raine T. IL-12， IL-23 and IL-17 in IBD： immunobiology and therapeutic targeting［J］. Nat Rev Gastroenterol HePatol， 2019， 16（3）：185-196.
Maloy KJ， Kullberg MC. IL-23 and Th17 cytokines in intestinal homeostasis［J］. Mucosal Immunol， 2008， 1（5）：339-349.
Lin H， Honglang L， Weifeng L， et al. The mechanism of alopolysaccharide protecting ulceralive colitis［J］. Biomed Pharmacother， 2017， 88：145-150.
Chu XQ， Wang J， Chen GX， et al. Overexpression of microRNA-495 improves the intestinal mucosal barrier function by targeting STAT3 via inhibition of the JAK/STAT3 signaling pathway in a mouse model of ulcerative colitis［J］. Pathol Res Pract， 2018， 214（1）：151-162.
Liu C， Zhao Q， Zhong L， et al. Tibetan medicine Ershiwuwei Lvxue Pill attenuates collagen-induced arthritis via inhibition of JAK2/STAT3 signaling pathway［J］. J Ethnopharmacol， 2021， 270：113820.
Zhou GY， Yi YX， Jin LX， et al. The protective effect of juglanin on fructose-induced hepatitis by inhibiting inflammation and apoptosis through TLR4 and JAK2/STAT3 signaling Pathways in fructose-fed rats［J］. Biomed Pharmacother， 2016， 81：318-328.
You G， Cao H， Yan L， et al. MicroRNA-10a-3P mediates Th17/Treg cell balance and improves renal injury by inhibiting REG3A in lupus nephritis［J］. Int Immunopharmacol， 2020， 88：106891.
Parham C， Chirica M， Timans J， et al. A receptor for the heterodimeric cytokine IL-23 is composed of IL-12Rbeta1 and a novel cytokine receptor subunit， IL-23R［J］. J Immunol， 2002， 168（11）：5699-5708.
Zhang M， Xu M， Wang K， et al. Effect of inhibition of the JAK2/STAT3 signaling pathway on the Th17/IL-17 axis in acute cellular rejection after heart transplantation in mice［J］. J Cardiovasc Pharmacol， 2021， 77（5）：614-620.
Feng X， Yang Q， Wang C， et al. Punicalagin exerts protective effects against ankylosing spondylitis by regulating NF-κB-TH17/JAK2/STAT3 signaling and oxidative stress［J］. Biomed Res Int， 2020， 2020：4918239.
Ding P， Liu J， Li Q， et al. Investigation of the active ingredients and mechanism of hudi enteric-coated capsules in DSS-induced ulcerative colitis mice based on network pharmacology and experimental verification ［J］. Drug Des Devel Ther， 2021， 15：4259-4273.
Zhu J， Yang T， Tang M， et al. Studies on the anti-psoriasis effects and its mechanism of a dual JAK2/FLT3 inhibitor flonoltinib maleate ［J］. Biomed Pharmacother， 2021， 137：111373.
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