嗜粘蛋白阿克曼氏菌通过调控CB<sub>2</sub>R缓解肠易激综合征大鼠内脏高敏

肖琳; 刘琴; 熊理守

doi:10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).20230418.001

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嗜粘蛋白阿克曼氏菌通过调控CB₂R缓解肠易激综合征大鼠内脏高敏

基础研究 | 更新时间：2024-02-19

- 嗜粘蛋白阿克曼氏菌通过调控CB₂R缓解肠易激综合征大鼠内脏高敏
- Akkermansia Muciniphila Alleviates Visceral Hypersensitivity in Irritable Bowel Syndrome Rats via Regulating CB₂R
- 中山大学学报（医学科学版） 2023年44卷第3期页码：379-385
- 作者机构：
  
  中山大学附属第一医院消化内科，广东广州 510080
- 作者简介：
  
  肖琳，硕士，研究方向：肠易激综合征的机制研究，E-mail：xiaolintemplee@163.com
- 基金信息：
  
  国家自然科学基金(81970471)
- DOI：10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).20230418.001
  中图分类号： R57
- 纸质出版日期：2023-05-20，
  
  收稿日期：2023-02-27，
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肖琳,刘琴,熊理守.嗜粘蛋白阿克曼氏菌通过调控CB₂R缓解肠易激综合征大鼠内脏高敏[J].中山大学学报（医学科学版）,2023,44(03):379-385.

XIAO Lin,LIU Qin,XIONG Li-shou.Akkermansia Muciniphila Alleviates Visceral Hypersensitivity in Irritable Bowel Syndrome Rats via Regulating CB₂R[J].Journal of Sun Yat-sen University(Medical Sciences),2023,44(03):379-385.
肖琳,刘琴,熊理守.嗜粘蛋白阿克曼氏菌通过调控CB₂R缓解肠易激综合征大鼠内脏高敏[J].中山大学学报（医学科学版）,2023,44(03):379-385. DOI： 10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).20230418.001.

XIAO Lin,LIU Qin,XIONG Li-shou.Akkermansia Muciniphila Alleviates Visceral Hypersensitivity in Irritable Bowel Syndrome Rats via Regulating CB₂R[J].Journal of Sun Yat-sen University(Medical Sciences),2023,44(03):379-385. DOI： 10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).20230418.001.

摘要

目的

探讨嗜粘蛋白阿克曼氏菌在新生期母婴分离（MS）和避水应激（WAS）肠易激综合征（IBS）大鼠模型的内脏高敏发生中的作用。

方法

将SD雄性大鼠在新生期随机分为3组：sham WAS（空白组），MS+WAS组（IBS模型组）及阿克曼氏菌组（

Akkermansia muciniphila， A. muciniphila

组），IBS模型组及阿克曼氏菌组采用母婴分离联合避水应激方法（MS+WAS）建立内脏高敏感模型。母婴分离刺激结束后，阿克曼氏菌组每天避水应激处理同时灌胃1 mL 1×10

CFU/mL

A. muciniphila

连续10 d。再用行为学观察和腹部回缩反射评分来检测内脏痛反应。

结果

经阿克曼氏菌干预后，实验组大鼠与模型组相比，体质量显著增加；粪便颗粒数及其不成形率显著下降；内脏疼痛阈值则显著上升。阿克曼氏菌组大鼠结肠黏膜内2型大麻素受体（CB

R）mRNA表达相比模型组显著上升。

结论

阿克曼氏菌可能通过调控结肠的CB

RmRNA表达来缓解IBS大鼠的内脏高敏。

Abstract

Objective

To investigate the mechanism of

Akkermansia muciniphila

（

A. muciniphila

） regulating the visceral hypersensitivity in irritable bowel syndrome （IBS） rats induced by neonatal maternal separation （MS） and water avoidance stress （WAS）.

Methods

Neonatal male Sprague-Dawley rats were randomly divided into sham WAS group （blank group）， MS+WAS group （IBS model group） and

A. muciniphila

group. IBS model was established by MS combined with WAS in both IBS model group and

A. muciniphila

group. Meanwhile， the rats in the

A. muciniphila

group were given 1 mL 1×10

CFU/mL

A. muciniphila

by gavage daily for 10 days. Visceral pain responses were detected by behavioral observations and abdominal withdrawal reflex scores.

Results

Compared with IBS model group，

A. muciniphila

group exhibited significant increase of body weight and visceral pain threshold， significantly decreased numbers of fecal particles and proportions of unformed stools， significantly higher expression levels of cannabinoid receptor type 2 （CB

R） mRNA in colon tissues.

Conclusion

A. muciniphila

may alleviate the visceral hypersensitivity in IBS rats by regulating the expression of CB

R mRNA in colonic tissues.

关键词

阿克曼氏菌2型大麻素受体肠易激综合征内脏高敏

Keywords

Akkermansia muciniphila （A. muciniphila）cannabinoid receptor type 2 （CB2R）irritable bowel syndrome （IBS）visceral hypersensitivity

references

Elsenbruch S. Abdominal pain in Irritable Bowel Syndrome： A review of putative psychological， neural and neuro-immune mechanisms［J］. Brain， Behavior， and Immunity，2011，25（3）：386-394.

Farzaei MH， Bahramsoltani R， Abdollahi M， et al. The Role of Visceral Hypersensitivity in Irritabl Bowel Syndrome： Pharmacological Targets and novel Treatments［J］. J Neurogastroenterol Motil，2016，4（22）：558-574.

Lucarini E， Di Pilato V， Parisio C， et al. Visceral sensitivity modulation by faecal microbiota transplantation： the active role of gut bacteria in pain persistence［J］. Pain，2022，163（5）：861-877.

Bie B， Wu J， Foss JF， et al. An overview of the cannabinoid type 2 receptor system and its therapeutic potential［J］. Current Opinion in Anaesthesiology，2018，31（4）：407-414.

Iwata Y， Ando K， Taniguchi K， et al. Identification of a highly potent and selective CB2 agonist， RQ-00202730， for the treatment of irritable bowel syndrome［J］. Bioorganic & Medicinal Chemistry Letters，2015，25（2）：236-240.

Collado MC， Derrien M， Isolauri E， et al. Intestinal Integrity andAkkermansia muciniphila， a Mucin-Degrading Member of the Intestinal Microbiota Present in Infants， Adults， and the Elderly［J］. Applied and Environmental Microbiology，2007，73（23）：7767-7770.

Everard A， Belzer C， Geurts L， et al. Cross-talk betweenAkkermansia muciniphila and intestinal epithelium controls diet-induced obesity［J］. Proceedings of the National Academy of Sciences，2013，110（22）：9066-9071.

Depommier C， Everard A， Druart C， et al. Serum metabolite profiling yields insights into health promoting effect of A. muciniphila in human volunteers with a metabolic syndrome［J］. Gut Microbes，2021，13（1）：1994270.

Rigsbee L， Agans R， Shankar V， et al. Quantitative Profi ling of Gut Microbiota of Children With Diarrhea-Predominant Irritable Bowel Syndrome［J］. Am J Gastroenterol，2012，11（107）：1740-1751.

Chen Y， Xiao S， Gong Z， et al. Wuji Wan Formula Ameliorates Diarrhea and Disordered Colonic Motility in Post-inflammation Irritable Bowel Syndrome Rats by Modulating the Gut Microbiota［J］. Frontiers in Microbiology，2017，8.

Cruz-Aguliar RM， Wantia N， Clavel T， et al. An Open-Labeled Study on Fecal Microbiota Transfer in Irritable Bowel Syndrome Patients Reveals Improvement in Abdominal Pain Associated with the Relative Abundance of Akkermansia Muciniphila［J］. Digestion，2019，100（2）：127-138.

Ren T， Wu J， Yew D， et al. Effects of neonatal maternal separation on neurochemical and sensory response to colonic distension in a rat model of irritable bowel syndrome［J］. American Journal of Physiology-Gastrointestinal and Liver Physiology，2007，292（3）：G849-G856.

Ford A C， Sperber AD， Corsetti M， et al. Irritable bowel syndrome［J］. The Lancet，2020，396（10263）：1675-1688.

Zhao L， Huang Y， Lu L， et al. Saturated long-chain fatty acid-producing bacteria contribute to enhanced colonic motility in rats［J］. Microbiome，2018，6（1）：107.

Zhou C， Fang X， Xu J， et al. Bifidobacterium longum alleviates irritable bowel syndrome-related visceral hypersensitivity and microbiota dysbiosis via Paneth cell regulation［J］. Gut Microbes，2020，12（1）：1782156.

Gwee K， Lu C， Ghoshal UC. Epidemiology of irritable bowel syndrome in Asia： something old， something new， something borrowed［J］. J Gastroenterol Hepatol，2009，10（24）：1601-1607.

Maxion-Bergemann S， Thielecke F， Abel F， et al. Costs of Irritable Bowel Syndrome in the UK and US［Z］. Auckland：Adis International，2006，24：21-37.

Kanazawa M， Palsson OS， Thiwan SIM， et al. Contributions of Pain Sensitivity and Colonic Motility to IBS Symptom Severity and Predominant Bowel Habits［J］. The American Journal of Gastroenterology，2008，103（10）：2550-2561.

Wang J， Zhang G， Yang Y， et al. Schisandra chinensis Lignans Exert Antidepressant Effects by Promoting BV2 Microglia Polarization toward the M2 Phenotype through the Activation of the Cannabinoid Receptor Type-2–Signal Transducer and Activator of Transcription 6 Pathway［J］. Journal of Agricultural and Food Chemistry，2022，70（44）：14157-14169.

Losa M， Manz SM， Schindler V， et al. Increased visceral sensitivity， elevated anxiety， and depression levels in patients with functional esophageal disorders and non‐erosive reflux disease［J］. Neurogastroenterology & Motility，2021，33（9）：e14177.

Kang CS， Ban M， Choi E J， et al. Extracellular vesicles derived from gut microbiota， especially Akkermansia muciniphila， protect the progression of dextran sulfate sodium-induced colitis［J］. PLoS One，2013，8（10）：e76520.

Bian X， Wu W， Yang L， et al. Administration of Akkermansia muciniphila ameliorates dextran sulfate sodium-induced ulcerative colitis in mice［J］. Frontiers in Microbiology，2019，10：2259.

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嗜粘蛋白阿克曼氏菌通过调控CB2R缓解肠易激综合征大鼠内脏高敏

Akkermansia Muciniphila Alleviates Visceral Hypersensitivity in Irritable Bowel Syndrome Rats via Regulating CB2R

DOI：10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).20230418.001

摘要

Abstract

关键词

Keywords

references

嗜粘蛋白阿克曼氏菌通过调控CB₂R缓解肠易激综合征大鼠内脏高敏

Akkermansia Muciniphila Alleviates Visceral Hypersensitivity in Irritable Bowel Syndrome Rats via Regulating CB₂R