中山大学孙逸仙纪念医院麻醉科,广东 广州 510120
刘玲,第一作者,研究方向:老年患者术后谵妄机制及防治,E-mail:liuling23@mail.sysu.edu.cn
纸质出版日期:2024-03-20,
收稿日期:2023-11-07,
录用日期:2024-02-21
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刘玲,刘付宁.鼠李糖乳杆菌对老年小鼠术后海马区小胶质细胞激活及Tau蛋白磷酸化的影响[J].中山大学学报(医学科学版),2024,45(02):226-232.
LIU Ling,LIU Funing.Effects of Lactobacillus Rhamnosus GG on Activated Microglia and Tau Phosphorylation in the Hippocampus of Aged Mice Induced by Anesthesia and Surgery[J].Journal of Sun Yat-sen University(Medical Sciences),2024,45(02):226-232.
刘玲,刘付宁.鼠李糖乳杆菌对老年小鼠术后海马区小胶质细胞激活及Tau蛋白磷酸化的影响[J].中山大学学报(医学科学版),2024,45(02):226-232. DOI: 10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).20240305.006.
LIU Ling,LIU Funing.Effects of Lactobacillus Rhamnosus GG on Activated Microglia and Tau Phosphorylation in the Hippocampus of Aged Mice Induced by Anesthesia and Surgery[J].Journal of Sun Yat-sen University(Medical Sciences),2024,45(02):226-232. DOI: 10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).20240305.006.
目的
2
探讨术前益生菌鼠李糖乳杆菌(LGG)灌胃对麻醉手术老年小鼠海马区小胶质细胞及Tau磷酸化的影响。
方法
2
18月龄C57BL/6J小鼠30只随机分为3组,10只/组:对照组,麻醉手术组,麻醉手术+鼠李糖乳杆菌组。生理盐水/LGG 10
9
CFU 150 μL灌胃,每日 1 次,连续20 d后接受异氟醚麻醉+剖腹探查手术,术后12 h免疫荧光染色检测海马区小胶质细胞激活状态,ELISA 检测IL-6的浓度变化,Western blot 检测Tau蛋白磷酸化位点Tau-pS202/pT205和total Tau蛋白表达变化。
结果
2
对照组海马区小胶质细胞呈静息状态,炎症因子IL-6浓度为(82.08±12.07) pg/mL。与对照组相比,麻醉手术组海马区小胶质细胞活化增生,胞体变大,突起缩短变粗,炎症因子IL-6上升至(123.7±5.72) pg/mL(
P
=0.000),磷酸化Tau-pS202/pT205蛋白表达量也明显增加(
P
=0.002)。而与麻醉手术组相比,麻醉手术+LGG组海马区小胶质细胞增生肥大不明显,炎症因子IL-6分泌减少至(96.68±9.59) pg/mL(
P
=0.008),磷酸化Tau-pS202/pT205蛋白表达量明显下降(
P
=0.002)。而3组total Tau蛋白表达水平差异无统计学意义。
结论
2
术前服用益生菌鼠李糖乳杆菌减轻麻醉手术导致的老年小鼠海马区小胶质细胞活化、炎症因子分泌增加、以及Tau蛋白磷酸化水平增加。
Objective
2
To investigate the effects of
Lactobacillus
rhamnosus
GG (LGG)on microglia and Tau phosphorylation in the hippocampus of aged mice induced by anesthesia and surgery.
Methods
2
A total of thirty 18-month-old C57BL/6J mice were randomly divided into three groups: control group, anesthesia surgery group, and anesthesia surgery + LGG group (10 mice/group). The aged mice were oral administered by NS or LGG 10
9
CFU 150 μL once a day for 20 days. Then anesthesia surgery group and anesthesia surgery +LGG group received anesthesia with isoflurane and exploratory laparotomy. The activation status of microglia in the hippocampus was detected by immunofluorescence staining 12 hours after surgery. IL-6 concentration changes was detected by ELISA. The expression changes of Tau protein phosphorylation site (Tau-pS202/pT205) and total Tau protein was detected by western blot.
Results
2
The microglia in the hippocampus of the control group were in a resting state, and the concentration of inflammatory factor IL-6 was (82.08 ± 12.07) pg/mL in control group. Compared to the control group, the anesthesia surgery group showed microglial cell Microglia were activated, the concentration of inflammatory factors IL-6 increased significantly to (123.7±5.72) pg/mL (
P
=0.000), and the expression of phosphorylated Tau-pS202/pT205 increased the hippocampus (
P
=0.002). Compared to the anesthesia surgery group, the activated microglia were inhibited, the concentration of IL-6 decreased to (96.68±9.59) pg/mL (
P
=0.008), and the expression of phosphorylated Tau-pS202/pT205 reduced significantly in the AS+LGG group (
P
=0.002). While there were no significant changes in total Tau protein among 3 groups.
Conclusion
2
Preoperative administration of probiotic LGG can alleviate the activation of microglia, increased secretion of inflammatory factors, and increased Tau protein phosphorylation levels in the hippocampus of elderly mice caused by anesthesia surgery.
鼠李糖乳杆菌老年小鼠小胶质细胞海马Tau蛋白磷酸化
lactobacillus rhamnosus GGaged micemicrogliahippocampusTau protein phosphorylation
Wang X, Sun G, Feng T, et al. Sodium oligomannate therapeutically remodels gut microbiota and suppresses gut bacterial amino acids-shaped neuroinflammation to inhibit Alzheimer's disease progression[J]. Cell Res, 2019, 29(10): 787-803.
Zhan G, Hua D, Huang N, et al. Anesthesia and surgery induce cognitive dysfunction in elderly male mice: the role of gut microbiota[J]. Aging (Albany NY), 2019, 11(6): 1778-1790.
Zhang Y, Baldyga K, Dong Y, et al. The association between gut microbiota and postoperative delirium in patients[J]. Transl Psychiatry, 2023, 13(1): 156.
Liufu N, Liu L, Shen S, et al. Anesthesia and surgery induce age-dependent changes in behaviors and microbiota[J]. Aging (Albany NY), 2020, 12: 1965-1986.
Zhao H, Wang Q, Cheng X, et al. Inhibitive effect of resveratrol on the inflammation in cultured astrocytes and microglia induced by Aβ1-42[J]. Neuroscience, 2018, 379: 390.
Mu JL, Liu XD, Dong YH, et al. Peripheral interleukin-6-associated microglial QUIN elevation in basolateral amygdala contributed to cognitive dysfunction in a mouse model of postoperative delirium[J]. Front Med (Lausanne), 2022, 9: 998397.
高昕, 杜冰滢, 陈晶晶, 等. 神经胶质细胞在血管性认知障碍发病机制中的研究进展[J]. 重庆医科大学学报, 2022, 47(7): 753-757.
Gao X, Du BY, Chen JJ, et al. Research progress of glial cells in the pathogenesis of vascular cognitive impairment[J]. J Chongqing Med Univ, 2022, 47(7): 753-757.
Yu L, Wen G, Zhu S, et al. Abnormal phosphorylation of tau protein and neuroinflammation induced by laparotomy in an animal model of postoperative delirium[J]. Exp Brain Res, 2021, 239(3): 867-880.
Zhang ZJ, Zheng XX, Zhang XY, et al. Aging alters Hv1-mediated microglial polarization and enhances neuroinflammation after peripheral surgery[J]. CNS Neurosci Ther, 2020, 26(3): 374-384.
Zhang H, Wei W, Zhao M, et al. Interaction between Aβ and tau in the pathogenesis of Alzheimer's disease[J]. Int J Biol Sci, 2021, 17(9): 2181-2192.
Le Freche H, Brouillette J, Fernandez-Gomez FJ, et al. Tau phosphorylation and sevoflurane anesthesia: an association to postoperative cognitive impairment[J]. Anesthesiology, 2012, 116(4): 779-787.
Sun M, Dong Y, Li M, et al. Dexmedetomidine and clonidine attenuate sevoflurane-induced tau phosphorylation and cognitive impairment in young mice via α-2 adrenergic receptor[J]. Anesth Analg, 2021, 132(3): 878-889.
Stefanoska K, Gajwani M, Tan ARP, et al. Alzheimer’s disease: ablating single master site abolishes tau hyperphosphorylation[J]. Sci Adv, 2022, 8(27): eabl8809.
Tan B, Babur E, Toy N, et al. Tau protein is differentially phosphorylated in young- and old-aged rats with experimentally induced hyperthyroidism [J]. Int J Dev Neurosci, 2022, 82(7): 654-663.
Rianda D, Agustina R, Setiawan, EA, et al. Effect of probiotic supplementation on cognitive function in children and adolescents: a systematic review of randomised trials[J]. Benef Microbes, 2019, 10(8): 873-882.
Chen JF, Zhuang Y, Jin SB, et al. Probiotic Lactobacillus rhamnosus GG (LGG) restores intestinal dysbacteriosis to alleviate upregulated inflammatory cytokines triggered by femoral diaphyseal fracture in adolescent rodent model[J]. Eur Rev Med Pharmacol Sci, 2021, 25(1): 376-389.
袁丽芳, 杨俊华, 邢志伟, 等. 口服益生菌改善铝诱发的小鼠海马炎症[J]. 中山大学学报(医学科学版), 2020, 41(2): 210-216.
Yuan LF, Yang JH, Xing ZW, et al. Oral probiotics improves aluminum-induced hippocampal inflammation in mice[J]. J Sun Yat-sen Univ (Med Sci), 2020, 41(2): 210-216.
Sadler R, Cramer JV, Heindl S, et al. Short-chain fatty acids improve poststroke recovery via immunological mechanisms[J]. J Neurosci, 2020, 40(5): 1162-1173.
Zhou X, Wu X, Wu Y, et al. Indole-3-propionic acid, a gut microbiota metabolite, protects against the development of postoperative delirium[J]. Ann Surg, 2023, 278(6):e1164-e1174.
Sun J, Xu J, Yang B, Chen K, et al. Effect of Clostridium butyricum against microglia-mediated neuroinflammation in Alzheimer's disease via regulating gut microbiota and metabolites butyrate[J]. Mol Nutr Food Res, 2020, 64(2): e1900636.
Zhang YY, Shen Y, Liu FN, et al. Transmission of Alzheimer’s disease-associated microbiota dysbiosis and its impact on cognitive function: evidence from mice and patients[J]. Mol Psychiatry, 2023, 28(10): 4421-4437.
Wang T, Yan H, Lu Y, et al. Anti-obesity effect of Lactobacillus rhamnosus LS-8 and Lactobacillus crustorum MN047 on high-fat and high-fructose diet mice base on inflammatory response alleviation and gut microbiota regulation[J]. Eur J Nutr, 2019, 59(6): 2709-2728.
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