1.暨南大学口腔医学院,广东 广州 510632
2.暨南大学附属第一医院口腔中心,广东 广州 510630
王越,第一作者,研究方向:正畸作用下口腔颌面组织抗菌机理研究,E-mail: 940955182@qq.com
纸质出版日期:2024-07-20,
收稿日期:2024-04-25,
录用日期:2024-06-13
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王越,林芳辰,苏琦等.黄芩素对MC3T3-E1体外成骨分化及对口腔常见菌的抑菌作用[J].中山大学学报(医学科学版),2024,45(04):602-612.
WANG Yue,LIN Fangchen,SU Qi,et al.Effect of Baicalein on Osteogenic Differentiation of MC3T3-E1 Cells and Its Bacteriostasis against Common Oral Bacteria[J].Journal of Sun Yat-sen University(Medical Sciences),2024,45(04):602-612.
王越,林芳辰,苏琦等.黄芩素对MC3T3-E1体外成骨分化及对口腔常见菌的抑菌作用[J].中山大学学报(医学科学版),2024,45(04):602-612. DOI: 10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).20240617.009.
WANG Yue,LIN Fangchen,SU Qi,et al.Effect of Baicalein on Osteogenic Differentiation of MC3T3-E1 Cells and Its Bacteriostasis against Common Oral Bacteria[J].Journal of Sun Yat-sen University(Medical Sciences),2024,45(04):602-612. DOI: 10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).20240617.009.
目的
2
研究不同浓度黄芩素对MCT3-E1增殖及生物学行为的影响,以及其对口腔常见菌的抑菌作用,并探讨其相关机制。
方法
2
将MC3T3-E1细胞分别培养在0、6、12、18、24 μmol/L的黄芩素浓度下,通过CCK-8实验检测黄芩素处理后MC3T3-E1的增殖活性;对成骨诱导培养后的MC3T3-E1进行ALP活性检测;RT-PCR法检测RunX2、BMP2、Osterix基因的表达差异。K-B纸片法检测黄芩素处理24 h对大肠杆菌,金黄色葡萄球菌,血链球菌的抑菌效果。
结果
2
黄芩素可在一定程度降低细胞增殖活力,但不影响其继续增殖。18 μmol/L的黄芩素可以提高MC3T3-E1的ALP活性,并有效上调BMP2与Osterix的表达,下调RunX2的表达,能有效抑制金黄色葡萄球菌与血链球菌的增殖(
P
<
0.05)。
结论
2
适宜浓度的黄芩素(18 μmol/L)对MC3T3-E1细胞成骨分化有促进作用,并且可有效抑制口腔常见菌(金黄色葡萄球菌与血链球菌)的增殖。
Objective
2
To investigate the impact of varying concentrations of baicalein on the proliferation and biological responses of MC3T3-E1 cells, as well as the antibacterial efficacy of baicalein against prevalent oral bacteria, and to elucidate the underlying mechanisms.
Methods
2
MC3T3-E1 cells were exposed to different concentrations of baicalein (0, 6, 12, 18, and 24 μmol/L) and cell viability was determined by using the CCK-8 assay. Alkaline phosphatase (ALP) activity of MC3T3-E1 cells following osteogenic induction was assessed. RT-PCR was used to examine the expression of RunX2, BMP2, and Osterix. After 24 hours of treatment, the antibacterial potential of baicalein against
Escherichia coli
,
Staphylococcus Aureus
and
Streptococcus Sanguis
was evaluated by using the K-B paper disk method.
Results
2
Baicalein exhibited a modest reduction in proliferation of MC3T3-E1 cells but without affecting their sustained proliferation. Baicalein at a concentration of 18 μmol/L enhanced ALP activity of MC3T3-E1 cells, upregulated BMP2 and Osterix expression, downregulated RunX2 expression, significantly inhibited the proliferation of
Staphylococcus Aureus
and
Streptococcus Sanguis
(
P
<
0.05).
Conclusions
2
Baicalein at an optimal concentration (18 μmol/L) demonstrated a promotional effect on the osteogenic differentiation of MC3T3-E1 cells and effectively suppressed the proliferation of common oral bac
teria, including
Staphylococcus Aureus
and
Streptococcus Sanguis
.
前成骨细胞黄芩素成骨作用抑菌分化增殖
preosteoblastsbaicaleinosteogenesisbacteriostasisdifferentiationproliferation
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