Morin Mediates Protective Effects on Bone in Aged Rats by Inhibiting ERK1/2-p38 Signaling Pathway

WANG Lin; ZHOU Maosheng

doi:10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2024.0322.002

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Morin Mediates Protective Effects on Bone in Aged Rats by Inhibiting ERK1/2-p38 Signaling Pathway

Preclinic Research | 更新时间：2024-04-15

- Morin Mediates Protective Effects on Bone in Aged Rats by Inhibiting ERK1/2-p38 Signaling Pathway
- Journal of Sun Yat-sen University(Medical Sciences) Vol. 45, Issue 2, Pages: 261-267(2024)
- 作者机构：
  
  皖南医学院弋矶山医院创伤骨科，安徽芜湖 241000
- 作者简介：
  
  WANG Lin； E-mail： 53113653@qq.com
- 基金信息：
- DOI：10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2024.0322.002
  CLC： R580
- Published：20 March 2024，
  
  Received：20 December 2023，
  
  Accepted：17 March 2024
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王林,周茂生.桑黄素通过抑制ERK1/2-p38信号通路介导对老年大鼠骨骼保护作用[J].中山大学学报（医学科学版）,2024,45(02):261-267.

WANG Lin,ZHOU Maosheng.Morin Mediates Protective Effects on Bone in Aged Rats by Inhibiting ERK1/2-p38 Signaling Pathway[J].Journal of Sun Yat-sen University(Medical Sciences),2024,45(02):261-267.
王林,周茂生.桑黄素通过抑制ERK1/2-p38信号通路介导对老年大鼠骨骼保护作用[J].中山大学学报（医学科学版）,2024,45(02):261-267. DOI： 10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2024.0322.002.

WANG Lin,ZHOU Maosheng.Morin Mediates Protective Effects on Bone in Aged Rats by Inhibiting ERK1/2-p38 Signaling Pathway[J].Journal of Sun Yat-sen University(Medical Sciences),2024,45(02):261-267. DOI： 10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2024.0322.002.

摘要

目的

探讨桑黄素（SSS）治疗对老年大鼠骨代谢及骨量影响，并阐明其可能的作用机制。

方法

10只3月龄年轻雌性Sprague-Dawley（SD）大鼠和20只24月龄老年雌性SD大鼠随机分为3组，对照组（CON，10只年轻大鼠）、模型组（MOD，10只老年大鼠）和桑黄素组（SSS，10只老年大鼠）。在实验过程中，SSS组每日接受腹腔注射桑黄素（10 mg/kg）治疗。治疗为期12周，待治疗结束后使用Micro-CT、HE染色切片、血清学检测以及蛋白质印迹观察治疗效果以及可能的机制。

结果

治疗12周后，与MOD组相比，SSS组的大鼠骨小梁数量和密度得到明显的改善。SSS组左侧股骨BMD、Conn.D、Tb.N、Tb.Th和Tb.Sp较MOD组明显改善（

0.05）。治疗12周时，SSS组CTX-1、骨钙素、TRACP-5b 和PINP水平较MOD显著降低（

0.05）。和MOD组比较，SSS组的大鼠ERK1/2-p38信号通路显著抑制，ERK1/2和p38水平显著降低，比较有统计学差异（

0.05）。

结论

桑黄素通过抑制ERK1/2-p38信号通路和降低骨转换来介导对老年大鼠骨骼保护作用。

Abstract

Objective

To investigate the effects of morin treatment on bone metabolism and bone mass in aged rats， and to clarify the possible mechanism.

Methods

Ten young female Sprague-Dawley rats （3 months old） and 20 old female Sprague-Dawley rats （24 months old） were randomly divided into three groups： Control group （CON， 10 young rats）； Model group （MOD， 10 young rats）； 10 old rats and SangHuangSu Group （SSS， 10 old rats）. During the experiment， the SSS group received intraperitoneal injection of morin （10 mg / kg） daily. The treatment lasted for 12 weeks. After treatment， Micro-CT， HE stained sections， serological tests and Western blot were used to observe the treatment effect and possible mechanism.

Results

After 12 weeks of treatment， compared with MOD group， the number and density of bone trabeculae in SSS group were significantly improved. The BMD， Conn. D， Tb. N， Tb.Th and Tb.Sp of the left femur in the SSS group were significantly better than those in the MOD group（

0.05）. After 12 weeks of treatment， the levels of CTX-1， osteocalcin， TRACP-5b and PINP in SSS group were significantly lower than those in MOD group（

0.05）. Compared with the MOD group， the ERK1/2-p38 signal pathway was significantly inhibited and the levels of ERK1/2 and p38 were significantly decreased in the SSS group（

0.05）.

Conclusion

Morin pigment mediates the protective effect on the bones of aged rats by inhibiting the ERK1/2-p38 signaling pathway and reducing bone turnover.

关键词

桑黄素骨质疏松症ERK1/2-p38信号通路骨密度大鼠

Keywords

morinosteoporosisERK1/2-p38signaling pathwaybone mineral densityrat

references

Hwang JH， Park YS， Kim HS， et al. Yam-derived exosome-like nanovesicles stimulate osteoblast formation and prevent osteoporosis in mice［J］. J Control Release， 2023， 355： 184-198.

Wang LT， Chen LR， Chen KH. Hormone-related and drug-induced osteoporosis： a cellular and molecular overview［J］. Int J Mol Sci， 2023， 24（6）： 5814.

Gosset A， Pouillès JM， Trémollieres F. Menopausal hormone therapy for the management of osteoporosis［J］. Best Pract Res Clin Endocrinol Metab， 2021， 35（6）： 101551.

Tao ZS， Tao M， Zhou MS， et al. Niacin treatment prevents bone loss in iron overload osteoporotic rats via activation of SIRT1 signaling pathway［J］. Chem Biol Interact， 2023， 388： 110827.

Ma J， Ma Y， Liu X， et al. Gambogic acid inhibits osteoclast formation and ovariectomy-induced osteoporosis by suppressing the JNK， p38 and Akt signalling pathways［J］. Biochem J， 2015， 469（3）： 399-408.

Gopinath V. Osteoporosis［J］. Med Clin North Am， 2023， 107（2）： 213-225.

Tao ZS， Li TL， Wei S. Silymarin prevents iron overload induced bone loss by inhibiting oxidative stress in an ovariectomized animal model［J］. Chem Biol Interact， 2022， 366： 110168.

代学俊，岑蔼儿，张春梅，等. 骨质疏松症抑制人间充质干细胞的成骨分化［J］. 中山大学学报（医学科学版）， 2014， 35（5）： 723-729.

Dai JX， Cen AE， Zhang CM， et al. Osteoporosis inhibits osteogenic differentiation of human mesenchymal stem cells［J］. J SUN Yat-sen Univ （Med Sci）， 2014， 35（5）： 723-729.

Walker MD， Shane E. Postmenopausal osteoporosis［J］. N Engl J Med， 2023， 389（21）：1979-1991.

Sabri SA， Chavarria JC， Ackert-Bicknell C， et al. Osteoporosis： an update on screening， diagnosis， evaluation， and treatment［J］. Orthopedics， 2023， 46（1）： e20-e26.

刘亚东，卢小伟. miR-495-3p联合黄氏总皂苷对高糖诱导的成骨细胞增殖、凋亡的影响及其机制研究［J］. 安徽医药， 2021， 25 （4）： 834-839.

Liu YD， Lu XW. Effect of miR-495-3p combined with total saponins on hyperglycemia-induced osteoblast proliferation and apoptosis and its mechanism［J］. Anhui Med， 2021， 25 （4）： 834-839.

李敏，史晓林，许超，等. 右归丸抗骨质疏松症的中药化合物及靶点网络药理学作用机制［J］. 中国骨伤， 2020， 33 （10）： 933-937.

Li M， Shi XN， Xu C， et al. Pharmacological mechanism of action of traditional Chinese medicine compounds and target network of Yougui pills against osteoporosis［J］. Chin J Orthop Traumatol， 2020， 33 （10）： 933-937.

孟令仪，张晶莹，周博宇，等. 传统中草药对骨质疏松症治疗功效的研究进展［J］. 世界复合医学， 2019， 5（10）： 195-198.

Meng LY， Zhang JY， Zhou BY， et al. Research progress on the efficacy of traditional chinese herbal medicine in the treatment of osteoporosis［J］. World Comp Med， 2019， 5（10）： 195-198.

Lin Y， Shen X， Ke Y， et al. Activation of osteoblast ferroptosis via the METTL3/ASK1-p38 signaling pathway in high glucose and high fat （HGHF）-induced diabetic bone loss［J］. FASEB J， 2022， 36（3）： e22147.

Ma Y， Ge A， Zhu W， et al. Morin attenuates ovalbumin-induced airway inflammation by modulating oxidative stress-responsive MAPK signaling［J］. Oxid Med Cell Longev， 2016， 2016： 5843672.

Li XJ， Zhu Z， Han SL， et al. Bergapten exerts inhibitory effects on diabetes-related osteoporosis via the regulation of the PI3K/AKT， JNK/MAPK and NF-κB signaling pathways in osteoprotegerin knockout mice［J］. Int J Mol Med， 2016， 38（6）： 1661-1672.

Thouverey C， Caverzasio J. Suppression of p38α MAPK signaling in osteoblast lineage cells impairs bone anabolic action of parathyroid hormone［J］. J Bone Miner Res， 2016， 31（5）： 985-993.

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Related Institution

Department of Blood Transfusion， Guangzhou First People’s Hospital // the Second Affiliated Hospital of South China University of Technology

School of Medicine， South China University of Technology

Department of Parasitology， Zhongshan School of Medicine， Sun Yat-sen University

(Department of Neurology, The Third People＇s Hospital of Zhengzhou,,)

Department of Ultrasound Imaging，The First Affiliated Hospital，Sun Yat-sen University

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