Effect of Precocious Puberty on Glucose and Lipid Metabolism in Female Rats

LIN Xiaoya; HUANG Baojiang; ZHANG Jun; GUO Song; MA Huamei; LI Yanhong; DU Minlian; CHEN Qiuli

doi:10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.20240305.005

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Effect of Precocious Puberty on Glucose and Lipid Metabolism in Female Rats

Preclinic Research | 更新时间：2024-04-15

- Effect of Precocious Puberty on Glucose and Lipid Metabolism in Female Rats
- Journal of Sun Yat-sen University(Medical Sciences) Vol. 45, Issue 2, Pages: 233-242(2024)
- 作者机构：
  
  中山大学附属第一医院儿科，广东广州 510080
- 作者简介：
  
  CHEN Qiuli；E-mail：chenqiul@mail.sysu.edu.cn
- 基金信息：
- DOI：10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.20240305.005
  CLC： R725.8
- Published：20 March 2024，
  
  Received：06 November 2023，
  
  Accepted：21 February 2024
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林小雅,黄宝江,张军等.雌性大鼠性早熟对糖脂代谢的影响[J].中山大学学报（医学科学版）,2024,45(02):233-242.

LIN Xiaoya,HUANG Baojiang,ZHANG Jun,et al.Effect of Precocious Puberty on Glucose and Lipid Metabolism in Female Rats[J].Journal of Sun Yat-sen University(Medical Sciences),2024,45(02):233-242.
林小雅,黄宝江,张军等.雌性大鼠性早熟对糖脂代谢的影响[J].中山大学学报（医学科学版）,2024,45(02):233-242. DOI： 10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.20240305.005.

LIN Xiaoya,HUANG Baojiang,ZHANG Jun,et al.Effect of Precocious Puberty on Glucose and Lipid Metabolism in Female Rats[J].Journal of Sun Yat-sen University(Medical Sciences),2024,45(02):233-242. DOI： 10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.20240305.005.

摘要

目的

探究雌性大鼠性早熟对糖代谢和脂代谢的影响。

方法

将60只两天龄的雌性大鼠随机分为性早熟组和正常组两组，分别在5天龄时给予达那唑（300 μg/只）或溶剂大豆油进行皮下注射，从21天龄起每天观察大鼠的阴道开口状况。建模成功的两组按照阴道开口顺序分别于阴道开口后3 d、7周龄和12周龄进行处死，处死前空腹12 h。

方法

测量麻醉后的体重和鼻肛长，通过酶联免疫吸附试验测量葡萄糖、胰岛素、血脂、雌二醇、瘦素和脂联素的浓度，病理切片观察肾周脂肪、子宫和卵巢的变化。

结果

通过正常组和早熟组两组3个时间点对比，早熟组在阴道开口3 d时体质量和鼻肛长小于正常组，但是不影响后续生长发育，7周龄和12周龄时两组体质量和鼻肛长差异无统计学意义。两组的胰岛素浓度对比在阴道开口后3天内有统计学意义（

= 0.001），早熟组存在高胰岛素血症，且早熟组此时的肾周脂肪细胞增多，在7和12周龄差异无统计学意义，两组3个时间点的雌二醇、瘦素和脂联素浓度差异均无统计学意义，两组卵巢和子宫与体质量的比重差异无统计学意义。

结论

通过两组大鼠的对比，性早熟使机体提前跨越发育的时间窗，不能适应内环境的骤变，产生的一系列变化只是暂时性且可逆性，至成年期会恢复。

Abstract

Objective

To explore the effect of precocious puberty on glucose metabolism and lipid metabolism in female rats.

Methods

Sixty two-day-old female rats were randomly divided into 2 groups. When aged 5 days， the precocious puberty group and normal group were given a single subcutaneous injection of danazol and solvent soybean oil respectively. The vaginal opening of rats was monitored from their 21 days of age. After 12 hours of fasting， all successful modeling rats were randomly executed within 3 days after vaginal opening， when aged 7 and 12 weeks. Then we measured the rats’ body weight and length， determined the concentrations of glucose， insulin， blood lipids， estradiol， leptin and adiponectin with enzyme-linked immunosorbent assay and observed the pathological changes of perirenal fat， uterus and ovary.

Results

For body weight and length， rats in the precocious puberty group were smaller than those in the normal group within 3 days after vaginal opening， but which did not affect their subsequent growth and development， and there was no significant difference between the two groups at 7 and 12 weeks of age. Within 3 days after vaginal opening， insulin levels had significant difference between the two groups （

= 0.001）， the precocious group showed hyperinsulinemia and increased number of perirenal adipocytes. At three execution times， no significant difference was noted in estradiol， leptin and adiponectin levels between the two groups. The same was true in the ratios of ovary or uterus to body weight between the two groups.

Conclusions

Precocious puberty makes earlier onset of pubertal development and allows body maladaptation to the sudden changes of the internal environment. However， the changes due to precocious puberty are temporary and reversible， and they may become normal in adulthood.

关键词

性早熟糖代谢脂代谢胰岛素抵抗雌性大鼠

Keywords

precocious pubertyglucose metabolismlipid metabolisminsulin resistancefemale rat

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Department of Pediatrics，The Third Affiliated Hospital of Sun Yat-sen University

Department of Gynecological Oncology， Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University

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