Effect of Baicalein on Osteogenic Differentiation of MC3T3-E1 Cells and Its Bacteriostasis against Common Oral Bacteria

WANG Yue; LIN Fangchen; SU Qi; HUANG Xia; ZHOU Zhiying

doi:10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.20240617.009

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Effect of Baicalein on Osteogenic Differentiation of MC3T3-E1 Cells and Its Bacteriostasis against Common Oral Bacteria

Preclinic Research | 更新时间：2024-07-30

- Effect of Baicalein on Osteogenic Differentiation of MC3T3-E1 Cells and Its Bacteriostasis against Common Oral Bacteria
- Journal of Sun Yat-sen University(Medical Sciences) Vol. 45, Issue 4, Pages: 602-612(2024)
- 作者机构：
  
  1.暨南大学口腔医学院，广东广州 510632
  2.暨南大学附属第一医院口腔中心，广东广州 510630
- 作者简介：
  
  ZHOU Zhiying； E-mail： dentistchow@163.com
- 基金信息：
- DOI：10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.20240617.009
  CLC： R781.42
- Published：20 July 2024，
  
  Received：25 April 2024，
  
  Accepted：13 June 2024
- 稿件说明：
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王越,林芳辰,苏琦等.黄芩素对MC3T3-E1体外成骨分化及对口腔常见菌的抑菌作用[J].中山大学学报（医学科学版）,2024,45(04):602-612.

WANG Yue,LIN Fangchen,SU Qi,et al.Effect of Baicalein on Osteogenic Differentiation of MC3T3-E1 Cells and Its Bacteriostasis against Common Oral Bacteria[J].Journal of Sun Yat-sen University(Medical Sciences),2024,45(04):602-612.
王越,林芳辰,苏琦等.黄芩素对MC3T3-E1体外成骨分化及对口腔常见菌的抑菌作用[J].中山大学学报（医学科学版）,2024,45(04):602-612. DOI： 10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.20240617.009.

WANG Yue,LIN Fangchen,SU Qi,et al.Effect of Baicalein on Osteogenic Differentiation of MC3T3-E1 Cells and Its Bacteriostasis against Common Oral Bacteria[J].Journal of Sun Yat-sen University(Medical Sciences),2024,45(04):602-612. DOI： 10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.20240617.009.

摘要

目的

研究不同浓度黄芩素对MCT3-E1增殖及生物学行为的影响，以及其对口腔常见菌的抑菌作用，并探讨其相关机制。

方法

将MC3T3-E1细胞分别培养在0、6、12、18、24 μmol/L的黄芩素浓度下，通过CCK-8实验检测黄芩素处理后MC3T3-E1的增殖活性；对成骨诱导培养后的MC3T3-E1进行ALP活性检测；RT-PCR法检测RunX2、BMP2、Osterix基因的表达差异。K-B纸片法检测黄芩素处理24 h对大肠杆菌，金黄色葡萄球菌，血链球菌的抑菌效果。

结果

黄芩素可在一定程度降低细胞增殖活力，但不影响其继续增殖。18 μmol/L的黄芩素可以提高MC3T3-E1的ALP活性，并有效上调BMP2与Osterix的表达，下调RunX2的表达，能有效抑制金黄色葡萄球菌与血链球菌的增殖（

0.05）。

结论

适宜浓度的黄芩素（18 μmol/L）对MC3T3-E1细胞成骨分化有促进作用，并且可有效抑制口腔常见菌（金黄色葡萄球菌与血链球菌）的增殖。

Abstract

Objective

To investigate the impact of varying concentrations of baicalein on the proliferation and biological responses of MC3T3-E1 cells， as well as the antibacterial efficacy of baicalein against prevalent oral bacteria， and to elucidate the underlying mechanisms.

Methods

MC3T3-E1 cells were exposed to different concentrations of baicalein （0， 6， 12， 18， and 24 μmol/L） and cell viability was determined by using the CCK-8 assay. Alkaline phosphatase （ALP） activity of MC3T3-E1 cells following osteogenic induction was assessed. RT-PCR was used to examine the expression of RunX2， BMP2， and Osterix. After 24 hours of treatment， the antibacterial potential of baicalein against

Escherichia coli

，

Staphylococcus Aureus

and

Streptococcus Sanguis

was evaluated by using the K-B paper disk method.

Results

Baicalein exhibited a modest reduction in proliferation of MC3T3-E1 cells but without affecting their sustained proliferation. Baicalein at a concentration of 18 μmol/L enhanced ALP activity of MC3T3-E1 cells， upregulated BMP2 and Osterix expression， downregulated RunX2 expression， significantly inhibited the proliferation of

Staphylococcus Aureus

and

Streptococcus Sanguis

（

0.05）.

Conclusions

Baicalein at an optimal concentration （18 μmol/L） demonstrated a promotional effect on the osteogenic differentiation of MC3T3-E1 cells and effectively suppressed the proliferation of common oral bac

teria， including

Staphylococcus Aureus

and

Streptococcus Sanguis

关键词

前成骨细胞黄芩素成骨作用抑菌分化增殖

Keywords

preosteoblastsbaicaleinosteogenesisbacteriostasisdifferentiationproliferation

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