WANG Yue,LIN Fangchen,SU Qi,et al.Effect of Baicalein on Osteogenic Differentiation of MC3T3-E1 Cells and Its Bacteriostasis against Common Oral Bacteria[J].Journal of Sun Yat-sen University(Medical Sciences),2024,45(04):602-612.
WANG Yue,LIN Fangchen,SU Qi,et al.Effect of Baicalein on Osteogenic Differentiation of MC3T3-E1 Cells and Its Bacteriostasis against Common Oral Bacteria[J].Journal of Sun Yat-sen University(Medical Sciences),2024,45(04):602-612. DOI: 10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).20240617.009.
Effect of Baicalein on Osteogenic Differentiation of MC3T3-E1 Cells and Its Bacteriostasis against Common Oral Bacteria
To investigate the impact of varying concentrations of baicalein on the proliferation and biological responses of MC3T3-E1 cells, as well as the antibacterial efficacy of baicalein against prevalent oral bacteria, and to elucidate the underlying mechanisms.
Methods
2
MC3T3-E1 cells were exposed to different concentrations of baicalein (0, 6, 12, 18, and 24 μmol/L) and cell viability was determined by using the CCK-8 assay. Alkaline phosphatase (ALP) activity of MC3T3-E1 cells following osteogenic induction was assessed. RT-PCR was used to examine the expression of RunX2, BMP2, and Osterix. After 24 hours of treatment, the antibacterial potential of baicalein against
Escherichia coli
,
Staphylococcus Aureus
and
Streptococcus Sanguis
was evaluated by using the K-B paper disk method.
Results
2
Baicalein exhibited a modest reduction in proliferation of MC3T3-E1 cells but without affecting their sustained proliferation. Baicalein at a concentration of 18 μmol/L enhanced ALP activity of MC3T3-E1 cells, upregulated BMP2 and Osterix expression, downregulated RunX2 expression, significantly inhibited the proliferation of
Staphylococcus Aureus
and
Streptococcus Sanguis
(
P
<
0.05).
Conclusions
2
Baicalein at an optimal concentration (18 μmol/L) demonstrated a promotional effect on the osteogenic differentiation of MC3T3-E1 cells and effectively suppressed the proliferation of common oral bac
Tang B, Dong Y. Network pharmacology and bioinformatics analysis on the underlying mechanisms of baicalein against oral squamous cell carcinoma[J]. J Gene Med, 2023, 25(6): e3490.
Luo H, Yu Y, Liang M, et al. Efficacy identification and active compounds screening of topically administration of Scutellaria Radix in oral ulcer[J]. J Chromatogr B Analyt Technol Biomed Life Sci, 2023, 1215: 123571.
Hou F, Yu Z, Cheng Y, et al. Deciphering the pharmacological mechanisms of Scutellaria baicalensis Georgi on oral leukoplakia by combining network pharmacology, molecular docking and experimental evaluations[J]. Phytomedicine, 2022, 103: 154195.
Chen H, Xie S, Gao J, et al. Flavonoid baicalein suppresses oral biofilms and protects enamel hardness to combat dental caries[J]. Int J Mol Sci, 2022, 23(18): 10593.
Ming J, Zhuoneng L, Guangxun Z. Protective role of flavonoid baicalin from Scutellaria baicalensis in periodontal disease pathogenesis: a literature review[J]. Complement Ther Med, 2018, 38: 11-18.
Sun JY, Li DL, Dong Y, et al. Baicalin inhibits toll-like receptor 2/4 expression and downstream signaling in rat experimental periodontitis[J]. Int Immunopharmacol, 2016, 36: 86-93.
Pei Z, Wang B, Zhang F, et al. Response of human periodontal ligament cells to baicalin[J]. J Periodontol, 2014, 85(9): 1283-1290.
Ren M, Zhao Y, He Z, et al. Baicalein inhibits inflammatory response and promotes osteogenic activity in periodontal ligament cells challenged with lipopolysaccharides[J]. BMC Complement Med Ther, 2021, 21(1): 43.
Luo W, Wang CY, Jin L. Baicalin downregulates Porphyromonas gingivalis lipopolysaccharide-upregulated IL-6 and IL-8 expression in human oral keratinocytes by negative regulation of TLR signaling[J]. PloS One, 2012, 7(12): e51008.
Yu F, Xu N, Zhou Y, et al. Anti-inflammatory effect of paeoniflorin combined with baicalin in oral inflammatory diseases[J]. Oral Dis, 2019, 25(8): 1945-1953.
Cao Z, Li C, Zhu G. Inhibitory effects of baicalin on IL-1beta- induced MMP-1/TIMP-1 and its stimulated effect on collagen-I production in human periodontal ligament cells[J]. Eur J Pharmacol, 2010, 641(1): 1-6.
Cheng YH, Li LA, Lin P, et al. Baicalein induces G1 arrest in oral cancer cells by enhancing the degradation of cyclin D1 and activating AhR to decrease Rb phosphorylation[J]. Toxicol Appl Pharmacol, 2012, 263(3): 360-367.
Tuli HS, Aggarwal V, Kaur J, et al. Baicalein: a metabolite with promising antineoplastic activity[J]. Life Sci, 2020, 259: 118183.
Li B, Lu M, Jiang XX, et al. Inhibiting reactive oxygen species-dependent autophagy enhanced baicalein-induced apoptosis in oral squamous cell carcinoma[J]. J Natural Med, 2017, 71(2): 433-441.
Vimalraj S. Alkaline phosphatase: structure, expression and its function in bone mineralization[J]. Gene, 2020, 754: 144855.
Cai H, Zou J, Wang W, et al. BMP2 induces hMSC osteogenesis and matrix remodeling[J]. Mol Med Rep, 2021, 23(2): 125.
Liu Q, Li M, Wang S, et al. Recent advances of osterix transcription factor in osteoblast differentiation and bone formation[J]. Front Cell Dev Biol, 2020, 8: 601224.
Komori T. Whole aspect of Runx2 functions in skeletal development[J]. Int J Mol Sci, 2022, 23(10): 5776.
Yin Q, Wang J, Fu Q, et al. CircRUNX2 through has-miR-203 regulates RUNX2 to prevent osteoporosis[J]. J Cel Mol Med, 2018, 22(12): 6112-6121.
Komori T. Animal models for osteoporosis[J]. Eur J Pharmacol, 2015, 759: 287-294.
Moriishi T, Fukuyama R, Ito M, et al. Osteocyte network; a negative regulatory system for bone mass augmented by the induction of Rankl in osteoblasts and Sost in osteocytes at unloading[J]. PloS One, 2012, 7(6): e40143.
Kim HJ, Kim WJ, Ryoo HM. Post-translational regulations of transcriptional activity of RUNX2[J]. Mol Cells, 2020, 43(2): 160-167.
Zhang H, Liu YF, Yang JY. Application of metagenomics in the study of oral diseases based on the flora changes of oral-gut axis[J]. Chin J Stomatol Res (Elect Edi), 2022, 16(3): 194 -198.
Lafaurie GI, Sabogal MA, Castillo DM, et al. Microbiome and microbial biofilm profiles of peri-implantitis: a systematic review[J]. J Periodontol, 2017, 88(10): 1066-1089.
Colombo AV, Barbosa GM, Higashi D, et al. Quantitative detection of Staphylococcus aureus, Enterococcus faecalis and Pseudomonas aeruginosa in human oral epithelial cells from subjects with periodontitis and periodontal health[J]. J Med Microbiol, 2013, 62(Pt 10): 1592-1600.
Persson GR, Renvert S. Cluster of bacteria associated with peri-implantitis[J]. Clin Implant Dent Relat Res, 2014, 16(6): 783-793.
Furugen R, Hayashida H, Saito T. Porphyromonas gingivalis and Escherichia coli lipopolysaccharide causes resistin release from neutrophils[J]. Oral Diseases, 2013, 19(5): 479-483.
Mojtahedi H, Hossein-Khannazer N, Mahmoud Hashemi S, et al. Effects of lipopolysaccharide from porphyromonas gingivalis and escherichia coli on gene expression levels of toll-like receptors and inflammatory cytokines in human dental pulp stem cells[J]. Iran J Immunol, 2022, 19(3): 299-310.